The Effect of Chinese Herb Essence on Genes Expression During Atherogenesis

• Main Authors: Chang, Yung-Sheng, 張永昇
• Other Authors: Chang, Wen-Cheng
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/90433469987217229585

碩士 === 中國醫藥學院 === 醫學研究所 === 91 === Atherosclerosis is a chronic inflammatory disease. To determine the effect in lowing the formation of the atheroma and molecular mechanism of Chinese herb treatment, New Zealand White (NZW) rabbits were assigned randomly into ten dietary groups. The normal group was fed regular rabbit chow and the cholesterol group was fed a chow containing 0.5 % cholesterol, the probucol group, the lovastatin group, and five Chinese herb essence groups were fed the same diet as the control group plus those additives, respectively. Animals were scarified after 8 weeks feeding and aortas were used to extract total RNA. Real-time quantitative RT-PCR was used to determine five specific gene expressions during atherogensis. The results of cholesterol group show over-expression in those five genes. And, the gene expressions of Chinese herb essence groups are significantly decreased as compare to cholesterol group and almost identical to the normal group. These results show that the protective effect of Chinese herb extraction against hypercholesterolemic atherosclerosis is valid. Human cDNA microarray were also performed to identify specific genes expression between the regular rabbit chow and the cholesterol group. To determine the homology between rabbit and human, we further choose 57 identified rabbit genes to performe semi-quantitative RT-PCR. It is promising using human cDNA microarray to screen genes expression during atherogenesis.