Identification of cellular proteins interacting with nonstructural protein 5B of hepatitis C virus

• Main Authors: Liao mei huei, 廖美惠
• Other Authors: Cheng ju chien
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/41208568251750356359

碩士 === 中國醫藥學院 === 醫學研究所 === 91 === The hepatitis C virus (HCV) is a positive single-stranded RNA virus and is an important pathogen for non-A, non-B hepatitis. HCV infection may sequentially induce chronic hepatitis to cirrhosis and hepatocellular carcinoma. Until now, the mechanisms of HCV pathogenesis have not yet completely understood. The HCV NS5B has been shown recently to serve as replicase in HCV replication. Although NS5B specifically binds to the 3’-end of the HCV genome, it does not display template specificity by itself. These results indicate that other viral or cellular protein(s) may involve in the replication of HCV. Using yeast two-hybrid system, eight potential NS5B interacting cellular clones were selected. Two of the selected clones were further shown the interacting property by in vitro co-immunoprecipitation assay. Polypyrimidine binding protein (PTB) was shown to bind the 5’-untranslated region (UTR) and the 3’-end 98 nucleotide (X region) of HCV RNA. Both of these PTB-binding sites were thought to regulate HCV replication and translation. Here we show that HCV NS5B and PTB directly interact with each other in vitro. In addition, the interacting region(s) between NS5B and PTB were further determined.