Effects of silicone tubes filled with astragaloside on regeneration of severed rat sciatic nerves

• Main Authors: Chun-Yuan Cheng, 鄭均洹
• Other Authors: Guan-Wei Chen
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/30006017348074434675

碩士 === 中國醫藥學院 === 中西醫結合研究所 === 91 === Effect of astragaloside on Peripheral Nerve Regeneration Chun-Yuan Cheng, Yueh-Sheng Chena, Chien-Ju Liua, Abstract The present study provides in vivo trials of silicone rubber chambers filled with different concentrations of astragaloside (0, 25, 50, 100 μM) to bridge a 15 mm sciatic nerve defect in rats. Collagen was also filled in the chambers to prevent the astragaloside from leakage. Histological and electrophysiological techniques were used to evaluate the functional recovery of the nerve. At the conclusion of 8 weeks, animals from the group treated with the astragaloside , especially at the concentration of 50μM, had a higher rate (40%) of successful regeneration across the wide gap and a significantly larger number of myelinated axons. However, the high dose astragaloside (100 μM) completely reversed this positive effect of growth-promoting capability and inhibited nerve regeneration. Only 10% of the animals treated with the high dose astragaloside had regenerated cables within the silicone rubber chambers. The low dose astragalosie (25μM) and control groups are no growth. These results indicated that astragaloside could be involved in both positive and negative effects on regenerating nerves. Therefore, whether a proper dosage of astragaloside is used or not plays a critical factor in deciding if it can sustain nerve regeneration over long gaps. We used this Astragalus membranaceusn(AM) as the material of this study is according to the Buyang Huanwu Decoction, the component Astragalus membranaceusn (AM).is around 40%. (19) Review the literatures of astragaloside on peripheral nerve regeneration no direct evidence on this issue. The possible mechanism macrophage played the role. Macrophage induced degeneration process of the injuried nerve, the cytokine and some growth factor also recruit for the regeneration procedure. Key words: astragaloside ; Silicone rubber chamber; Peripheral nerve, silicone tube, astragaloside