Effects of silicone tubes filled with astragaloside on regeneration of severed rat sciatic nerves
碩士 === 中國醫藥學院 === 中西醫結合研究所 === 91 === Effect of astragaloside on Peripheral Nerve Regeneration Chun-Yuan Cheng, Yueh-Sheng Chena, Chien-Ju Liua, Abstract The present study provides in vivo trials of silicone rubber chambers filled with different concentrations of ast...
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ndltd-TW-091CMCH06870052015-10-13T16:56:28Z http://ndltd.ncl.edu.tw/handle/30006017348074434675 Effects of silicone tubes filled with astragaloside on regeneration of severed rat sciatic nerves 內填黃耆皂苷矽膠管對截斷大鼠坐骨神經再生之研究 Chun-Yuan Cheng 鄭均洹 碩士 中國醫藥學院 中西醫結合研究所 91 Effect of astragaloside on Peripheral Nerve Regeneration Chun-Yuan Cheng, Yueh-Sheng Chena, Chien-Ju Liua, Abstract The present study provides in vivo trials of silicone rubber chambers filled with different concentrations of astragaloside (0, 25, 50, 100 μM) to bridge a 15 mm sciatic nerve defect in rats. Collagen was also filled in the chambers to prevent the astragaloside from leakage. Histological and electrophysiological techniques were used to evaluate the functional recovery of the nerve. At the conclusion of 8 weeks, animals from the group treated with the astragaloside , especially at the concentration of 50μM, had a higher rate (40%) of successful regeneration across the wide gap and a significantly larger number of myelinated axons. However, the high dose astragaloside (100 μM) completely reversed this positive effect of growth-promoting capability and inhibited nerve regeneration. Only 10% of the animals treated with the high dose astragaloside had regenerated cables within the silicone rubber chambers. The low dose astragalosie (25μM) and control groups are no growth. These results indicated that astragaloside could be involved in both positive and negative effects on regenerating nerves. Therefore, whether a proper dosage of astragaloside is used or not plays a critical factor in deciding if it can sustain nerve regeneration over long gaps. We used this Astragalus membranaceusn(AM) as the material of this study is according to the Buyang Huanwu Decoction, the component Astragalus membranaceusn (AM).is around 40%. (19) Review the literatures of astragaloside on peripheral nerve regeneration no direct evidence on this issue. The possible mechanism macrophage played the role. Macrophage induced degeneration process of the injuried nerve, the cytokine and some growth factor also recruit for the regeneration procedure. Key words: astragaloside ; Silicone rubber chamber; Peripheral nerve, silicone tube, astragaloside Guan-Wei Chen Yueh-Sheng Chen 陳光偉 陳悅生 2003 學位論文 ; thesis 78 zh-TW |
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碩士 === 中國醫藥學院 === 中西醫結合研究所 === 91 === Effect of astragaloside on Peripheral Nerve Regeneration
Chun-Yuan Cheng, Yueh-Sheng Chena, Chien-Ju Liua,
Abstract
The present study provides in vivo trials of silicone rubber chambers filled with different concentrations of astragaloside (0, 25, 50, 100 μM) to bridge a 15 mm sciatic nerve defect in rats. Collagen was also filled in the chambers to prevent the astragaloside from leakage. Histological and electrophysiological techniques were used to evaluate the functional recovery of the nerve. At the conclusion of 8 weeks, animals from the group treated with the astragaloside , especially at the concentration of 50μM, had a higher rate (40%) of successful regeneration across the wide gap and a significantly larger number of myelinated axons. However, the high dose astragaloside (100 μM) completely reversed this positive effect of growth-promoting capability and inhibited nerve regeneration. Only 10% of the animals treated with the high dose astragaloside had regenerated cables within the silicone rubber chambers. The low dose astragalosie (25μM) and control groups are no growth. These results indicated that astragaloside could be involved in both positive and negative effects on regenerating nerves. Therefore, whether a proper dosage of astragaloside is used or not plays a critical factor in deciding if it can sustain nerve regeneration over long gaps.
We used this Astragalus membranaceusn(AM) as the material of this study is according to the Buyang Huanwu Decoction, the component Astragalus membranaceusn (AM).is around 40%. (19) Review the literatures of astragaloside on peripheral nerve regeneration no direct evidence on this issue. The possible mechanism macrophage played the role. Macrophage induced degeneration process of the injuried nerve, the cytokine and some growth factor also recruit for the regeneration procedure.
Key words: astragaloside ; Silicone rubber chamber; Peripheral nerve, silicone tube, astragaloside
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author2 |
Guan-Wei Chen |
author_facet |
Guan-Wei Chen Chun-Yuan Cheng 鄭均洹 |
author |
Chun-Yuan Cheng 鄭均洹 |
spellingShingle |
Chun-Yuan Cheng 鄭均洹 Effects of silicone tubes filled with astragaloside on regeneration of severed rat sciatic nerves |
author_sort |
Chun-Yuan Cheng |
title |
Effects of silicone tubes filled with astragaloside on regeneration of severed rat sciatic nerves |
title_short |
Effects of silicone tubes filled with astragaloside on regeneration of severed rat sciatic nerves |
title_full |
Effects of silicone tubes filled with astragaloside on regeneration of severed rat sciatic nerves |
title_fullStr |
Effects of silicone tubes filled with astragaloside on regeneration of severed rat sciatic nerves |
title_full_unstemmed |
Effects of silicone tubes filled with astragaloside on regeneration of severed rat sciatic nerves |
title_sort |
effects of silicone tubes filled with astragaloside on regeneration of severed rat sciatic nerves |
publishDate |
2003 |
url |
http://ndltd.ncl.edu.tw/handle/30006017348074434675 |
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