| Summary: | 碩士 === 中國醫藥學院 === 中西醫結合研究所 === 91 === Abstract :
BACKGROUND : Electrical stimulation of meridian points in the rat inhibits the nociceptive tail withdrawal reflex. It’s pain mechanisms are well documented, but the changes of extracellular glucose and lactate metabolites at cellular level are still unclear. The aim of the study was to evaluate these changes using a rat model in combination with the microdialysis technique for the analysis of extracellular neurochemicals. METHODS: Electrical stimulation was applied in anaesthetized intact rats (n =7) with 2 ms square pulses, 2Hz at the Zusanli points. A second electrical stimulation (2-Hz pulses) was delivered in 2 of them in the same spot. A second electrical stimulation of a different pulse (100-Hz pulses) was delivered. in another 1 in the same spot. A second electrical stimulation of 2-Hz pulses was delivered in another 3 rats in non-acpoints to compare the difference due to the different locations of stimulation. The stimulation lasted for 30 min each time it was delivered. A 90-min recovery period was allowed between the first electrical stimulation and the later treatment to avoid interruption. An automated micro-blood sample collector was used for examining the glucose, pyruvate and lactate value. CONCLUSIONS: electro-acupunctural signal has an influence on the biologic process of energy metabolism by mediating dynamic extracellular neuro-chemical changes. EA on limb acupoints of lower limbs could induce obvious decrease in glucose, increases in lactate metabolites and decrease in lactate/glucose ratio. Moreover, the high lactate/glucose ratio suggests that the cell have an increased anaerobic glucose metabolism.
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