| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 91 === From the ethanol extract of Glossogyne tenuifolia Cass., six compounds were
isolated: oleanolic acid（1）, luteolin-7-glucoside（2）, b-sitosterol（3）,
stigmasterol（4）, stearic acid （5）and palmitic acid（6）.The isolated
compounds were identified based on the spectral analysis. Of these isolated
compounds, 3, 4, 5 and 6 were isolated for the first time from G. tenuifolia
Cass.
In order to demonstrate the possible inhibitory effect of G. tenuifolia on
production of HBsAg（Hepatitis B surface antigen）, PLC/PRF/5, a human
hepatoma cell line which secrets high level of HBsAg in the medium, was used
in vitro test. The ethanol extract of G. tenuifolia Cass. (0.2 mg/ml)
inhibited HBsAg secretion in a dose dependent manner without any cytotoxicity.
Among the partition layers, the interface marc between n-hexane and aqueous
methanol showed the strongest inhibition on HBsAg production with significant
cytotoxicity. Although the ethyl acetate layer as well as the marc between n-
buthanol and water partition and n-hexane layer exhibited inhibition on HBsAg
production, they showed no cytotoxicity. The other partition layers had no
effect on HBsAg production. Two compounds, luteolin-7-glucoside and oleanolic
acid, isolated from the potent inhibitory partition layers, showed no
inhibition on HBsAg production. Combination of luteolin-7-glucoside and
oleanolic acid showed no inhibition on HBsAg production, either. This result
indicates that oleanolic acid and luteolin-7-glucoside are the major
constitutes of G. tenuifolia, rather than active components responsible for
inhibition of HBsAg production.
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