Studies on the Chemical Constituents of Glossogyne tenuifolia Cass. and Its Effcet on HBsAg production in PLC/PRF/5 Hepatoma cells

• Main Authors: Pei-Jung Wu, 吳佩蓉
• Other Authors: Hsiou - Yu Ding
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/91256953322585951193

碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 91 === From the ethanol extract of Glossogyne tenuifolia Cass., six compounds were isolated: oleanolic acid（1）, luteolin-7-glucoside（2）, b-sitosterol（3）, stigmasterol（4）, stearic acid （5）and palmitic acid（6）.The isolated compounds were identified based on the spectral analysis. Of these isolated compounds, 3, 4, 5 and 6 were isolated for the first time from G. tenuifolia Cass. In order to demonstrate the possible inhibitory effect of G. tenuifolia on production of HBsAg（Hepatitis B surface antigen）, PLC/PRF/5, a human hepatoma cell line which secrets high level of HBsAg in the medium, was used in vitro test. The ethanol extract of G. tenuifolia Cass. (0.2 mg/ml) inhibited HBsAg secretion in a dose dependent manner without any cytotoxicity. Among the partition layers, the interface marc between n-hexane and aqueous methanol showed the strongest inhibition on HBsAg production with significant cytotoxicity. Although the ethyl acetate layer as well as the marc between n- buthanol and water partition and n-hexane layer exhibited inhibition on HBsAg production, they showed no cytotoxicity. The other partition layers had no effect on HBsAg production. Two compounds, luteolin-7-glucoside and oleanolic acid, isolated from the potent inhibitory partition layers, showed no inhibition on HBsAg production. Combination of luteolin-7-glucoside and oleanolic acid showed no inhibition on HBsAg production, either. This result indicates that oleanolic acid and luteolin-7-glucoside are the major constitutes of G. tenuifolia, rather than active components responsible for inhibition of HBsAg production.