Expression and Characterization of A Novel 17-beta Hydroxysteroid Dehydrogenase of Human and Zebrafish

• Main Authors: Liang-Yi Lin, 林良怡
• Other Authors: Te-Chung Liu
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/16224509716232782021

碩士 === 中山醫學大學 === 營養科學研究所 === 91 === ABSTRACT In this study, by using BLAST (Basic Local Alignment Search Tool), a novel 17-beta Hydroxysteroid Dehydrogenase of zebra fish(accession number AI477544) and human(accession number NP_115679) were found from NCBI (National Center for Biotechnology Information) nucleotide database. The novel 17-beta Hydroxysteroid Dehydrogenase cDNA of zebra fish was successfully amplified from the 0-7 days old zebra fish embryo cDNA library by using the combination of PCR and 3’rapid-amplification of cDNA ends (3’RACE) methods. The novel zebra fish cDNA had a 1469 bp open reading frame and 416 deduced amino acid. The novel human cDNA had a 3303bp open reading frame and 345 amino acid. Both enzyme had two domain, i.e. short chain alcohol dehydrogenase(adh_short) and steroid carried protein(SCP2)domain. The initial velocity analysis of both enzyme showed that two substrates, Estriol and 5-Androstene 3β,16α,17β-triol may participate in the catalytic reaction of the novel 17-beta Hydroxysteroid Dehydrogenase.