Optimization of Lipase-catalyzed Specialty Lipids 1,3-dicapryloyl-2-palmitoyl-sn- Glycerol

• Main Authors: Chun-Min Chen, 陳純敏
• Other Authors: Chwen-Jen Shieh
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/51941158104739069834

碩士 === 大葉大學 === 食品工程學系碩士班 === 91 === Structured lipids have been developed for human milk fat replacer (HMF replacer) substitutes or improved absorption for nutritional application. In this study, transesterification acidolysis of tripalmitin and caprylic acid catalyzed by lipases Lipozyme® IM-77 from Rhizomucor miehei to produce structured lipids with palmitoyl moieties in the secondary (sn-2) and medium-chain acyl moieties in the primary (sn-1,3) positions (1,3-dicapryloyl-2-palmitoyl-sn-glycerol), which should be helpful products for premature infant nutrient. The reaction stage was simplified to achieve one-stage fractionation. Response surface methodology (RSM) and three-level-four-factor central composite rotatable design (CCRD) were employed to evaluate the effects of HMF replacer substitute synthesis on the parameters: reaction time (1-3 hrs.), temperature (35-55 ℃), substrate molar ratio (caprylic acid: tripalmitin =3:1-5:1), and enzyme amount(0.2-1 BAUN). To clarify the relationships between the factors and the response the contour plots analysis was used to determine the optimal conditions for HMF replacer synthesis. Based on the ridge of max analysis, the optimum conditions were: reaction time 2h, synthesis temperature 52.2 0C, enzyme amount 0.75 BAUN, and substrate molar ratio (caprylic acid: tripalmitin) 4.5: 1. At the optimum point, the optimized acid incorporation was 87%.