| Summary: | 碩士 === 高雄醫學大學 === 藥學研究所 === 91 === Abstract
In the nature, arsenic is one of the wide distribution toxic trace element and carcinogen. Recently, the arsenic trioxide was found to be an effective drug for leukemia. However, the direct detection of arsenic species in blood of living organisms treated with arsenic trioxide is still a challenge task. In the present study we try to develop an on-line analytical system to automatic, continuous, and dynamic speciation of arsenic in blood of living animal.
An on-line and fully automatic method was developed for the continuous and dynamic in vivo monitoring of four arsenic species [arsenite (AsIII), arsenate (AsV), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA)] in blood of living organisms treated with arsenic trioxide. Microdialysis sampling technique was employed to couple with high performance liquid chromatography (HPLC) and hydride generation atomic absorption spectrometry (HGAAS). Detection limits of 2.0, 2.9, 2.4 and 4.2 ng ml-1 were obtained for AsIII, MMA, DMA and AsV, respectively. Precision values of 7.0% (AsIII), 7.0% (MMA), 5.1% (DMA) and 8.7% (AsV) were obtained, respectively, at a 10 ng ml-1 level. Recoveries close to 100%, relative to an aqueous standard, were observed for each species. The average in vivo recoveries of AsIII, MMA, DMA and AsV in rabbit blood were 6.9 ± 1.4%, 12.7 ± 2.6%, 8.6 ± 2.0% and 11.6 ± 2.4%, respectively, at a 10 ng ml-1 level. The applicability of the proposed method permits the determination of four arsenic species in the blood of rabbits treated with arsenic trioxide with a temporal resolution of 20 min sampling.
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