Bioinformatics Web-based Primer Design System: @Primer

• Main Authors: Dai Hua, Lee, 李大華
• Other Authors: Eugenia Huang
• Format: Others
• Language: en_US
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/85390184424052886201

碩士 === 國立政治大學 === 資訊管理研究所 === 91 === The invention of PCR (Polymerase Chain Reaction) has revolutionized bioinformatics and molecular technology. PCR can amplify specific region of DNA sequence in few minutes. One of these critical parameters is the selection of short oligonucleotides, which called primer. To design the suitable primers is still not part of any standardized algorithm, and several different sets of rules or criteria have been proposed to control the selection of primer sequence. Our research results include the followings: we have created a general-purpose application that design for primer design with relative PCR domain knowledge. We have also implemented a prototype system with Microsoft new OOP language, C#. We also provide a simplify version of Multiple PCR, it could handle two DNA templates to produce qualified multiple primer sets. This program can scan entire gene sequences for all possible primer pairs obeying these primer design rules and displays information such as the length and melting temperature of each primer, which may be of additional help in choosing primers. This program allows the stringency of certain criteria to be varied for better suit the requirement of particular experiment. We try to provide a convenient computerized tool which has a friendly user interface for biologists or scientists to use easily. The design of our system is based on OOA (Object-Oriented Analysis) and OOP (Object-Oriented Programming). Therefore programmers can easily change some algorithm or updated biology data to improve the efficiency of our primer design system.