Analysis of the photosynthetic properties and protein patterns in the chlorosis mutant of oryza sativa L., cv. TNG67

• Main Author: 陳靜怡
• Other Authors: 顏宏真
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/40170843822041994892

碩士 === 國立中興大學 === 生命科學系 === 91 === The material used in this study is the chlorosis mutant of Oryza sativa L. cv. TNG67 called C6. The leaves of C6 mutant become yellow during growth. This experiment measures the photosynthetic rate, the activity of Rubisco and the contents of photosynthetic pigments of the C6 and TNG67 at different stages. The total leaf proteins were separated by 2-D electrophoresis, analyzed by MALDI-Q-TOF MS and identified the protein identifies in the protein databases. The leaves of C6 mutant became yellow at the 14th day after transplanting, and distinguished into yellow and green leaves at tillering stage. The height of C6 mutant was lower than that of TNG67, and the time of panicle initiation was delayed seven weeks. The panicles of C6 mutant were smaller and lighter than that of TNG67. The seed number of each panicle in C6 mutant was less, and the seeds were not fully filled. The net photosynthetic rate and the activity of Rubisco in the yellow leaves of C6 mutant was lower than those of TNG67. The contents of the photosynthetic pigments separated by paper chromatography were lower in the yellow leaves of C6 than C6 green leaves and TNG67 leaves. The chlorophyll a and b contents of the C6 mutant green leaves were similar to TNG67 leaves, but the carotenoid content was only 0.3 folds of TNG67 leaves. The total proteins extracted from the leaves of the C6 mutant and TNG67 at tillering stage and booting stage was separated by 2-D electrophoresis. Analysis of protein profiles showed that there were 15 protein spots exhibiting different accumulation patterns between C6 mutant and TNG67 at tillering stage, and 17 protein spots at booting stage. There were 6 protein spots exhibiting different accumulation patterns during development. Totally, 14 protein spots were analyzed by MALDI-Q-TOF MS, and sreached the protein identities by Mascot and ProteinProspector programs. Two protein spots identified was chloroplastic ATP synthase beta chain which was missing in C6 mutant, and superoxide dismutase [Cu-Zn] which was accumulated in higher level in C6 mutant. Using the physiological properties and proteomics techniques helps us to predict the positions where the mutations occurred. It is a useful way to characterize the mutants generated by chemical mutagenesis.