A New Normalization method for cDNA Microarray data
碩士 === 國立成功大學 === 統計學系碩博士班 === 91 === Microarray experiment involves many steps, and for each step there may exist systematic variation. In order to making correct decision, this systematic bias has to be identified and be removed before analyzing data. Based on the concept of lowess normalization p...
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ndltd-TW-091NCKU53370182016-06-22T04:13:47Z http://ndltd.ncl.edu.tw/handle/52459201763305882760 A New Normalization method for cDNA Microarray data cDNA微陣列資料的新normalization方法 Li-Ju Chen 陳麗如 碩士 國立成功大學 統計學系碩博士班 91 Microarray experiment involves many steps, and for each step there may exist systematic variation. In order to making correct decision, this systematic bias has to be identified and be removed before analyzing data. Based on the concept of lowess normalization process suggested by Dudoit et al. (2001), a more appropriate normalization method would be presented in this thesis to deal with genes with replicate expressions. Simulation study shows that the performance of the suggested normalization method is superior to the available approaches. We use a microarray data for bladder cancer supplied by National Cheng Kung University Hospital to illustrate the application, and use real-time PCR to evaluate the performance of our approach. Shin-Huang Chan 詹世煌 2003 學位論文 ; thesis 61 zh-TW |
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碩士 === 國立成功大學 === 統計學系碩博士班 === 91 === Microarray experiment involves many steps, and for each step there may exist systematic variation. In order to making correct decision, this systematic bias has to be identified and be removed before analyzing data. Based on the concept of lowess normalization process suggested by Dudoit et al. (2001), a more appropriate normalization method would be presented in this thesis to deal with genes with replicate expressions. Simulation study shows that the performance of the suggested normalization method is superior to the available approaches. We use a microarray data for bladder cancer supplied by National Cheng Kung University Hospital to illustrate the application, and use real-time PCR to evaluate the performance of our approach.
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author2 |
Shin-Huang Chan |
author_facet |
Shin-Huang Chan Li-Ju Chen 陳麗如 |
author |
Li-Ju Chen 陳麗如 |
spellingShingle |
Li-Ju Chen 陳麗如 A New Normalization method for cDNA Microarray data |
author_sort |
Li-Ju Chen |
title |
A New Normalization method for cDNA Microarray data |
title_short |
A New Normalization method for cDNA Microarray data |
title_full |
A New Normalization method for cDNA Microarray data |
title_fullStr |
A New Normalization method for cDNA Microarray data |
title_full_unstemmed |
A New Normalization method for cDNA Microarray data |
title_sort |
new normalization method for cdna microarray data |
publishDate |
2003 |
url |
http://ndltd.ncl.edu.tw/handle/52459201763305882760 |
work_keys_str_mv |
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