Genotoxicity Assessment of AirborneSemivolatile Organic Compounds Using Mutatox

• Main Authors: Li-Ping Chen, 陳立蘋
• Other Authors: Ta-Chang Lin
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/70815178095494795163

碩士 === 國立成功大學 === 環境工程學系碩博士班 === 91 === Seven airborne samples with varying degrees of contamination. The airborne samples of both particle phase and gas phase of SVOCs were collected using high-volume samplers (PS-1) to asses the genotoxicity of airbone SVOCs. Air samples at seven sites / environments, including a motorcycle tunnel, a gasoline engine tunnel, and a diesel bus parking lot, an urban site in Tainan, plus three incense sticks, were collelcted for genotoxicity assessment using Mutatox test both with and without activation with S-9. Ambient air at traffic sites, i.e. the gasoline engine tunnel and the motorcycle vehicle tunnel were found to have positive genotoxicity with or without S-9 activation. Extracts from diesel parking lot were found to have only suspected genotoxicity with or without S9 activation. Finally, the urban site was found to be negative genotoxic with or without S-9 activation. As to the smoke from incense burning, all the SOF of tested incense were found to be positive genotoxic after S-9 activation. The XOC samples of tested incense were found to have suspect genotoxic after S-9 activation. The genotoxicity of particle-associated (SOF) samples was generally higher than those in the vapor phase (XOC) extacts in all tested air pollutions. This study shows Mutatox assay is an excellent assessment tool for monitoring toxicity of air samples. This in vitro bioassay is fast, convenient, and cost-effective, and can be widely used as for preliminary toxicity screening of environmental samples.