nalysis of biogenic amines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence
碩士 === 國立交通大學 === 應用化學系 === 91 === Analysis of biogenic amines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence Student:Yi-Fang Chen Advisor: You-Zung Hsieh Department of Applied Chemistry National Chiao Tung University Abstract...
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ndltd-TW-091NCTU05000232016-06-22T04:14:27Z http://ndltd.ncl.edu.tw/handle/12649392964329096738 nalysis of biogenic amines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence 應用微胞電動層析-雷射誘發螢光偵測法檢測生物胺 Yi-Fang Chen 陳義芳 碩士 國立交通大學 應用化學系 91 Analysis of biogenic amines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence Student:Yi-Fang Chen Advisor: You-Zung Hsieh Department of Applied Chemistry National Chiao Tung University Abstract Mono- or polyamines involved in biological activities and that exist in nature are sometimes called by biogenic amines. Some of them are biologically synthesized from amino acids by amino acid decarboxylase degradation and play an important role in living systems. In this study, analysis of fourteen biogenic amines by micellar electrokinetic capillary electrophoresis and laser induce fluorescence were investigated. The selected derivatizing reagent was fluorescein isothiocyanate ( FITC). In order to achieve an optimum detection sensitivity and separation efficiencies,it was necessary to optimize precolumn derivatization conditions with respect to reaction time, pH, buffer concentration and the molar ratios of FITC to biogenic amines. It must also study the optimum separation conditions. In an optimized condition of micellar electrokinetic chromatography, using 40 mM sodium borate buffer (pH 10.1) with 40 mM sodium dodecyl sufate (SDS), fourteen biogenic amines could be successful separated. To check the applicability of the method, affective disorder patient’s urine was analyzed. We additional spiked E and NE to identify the existence of E and NE in the urine. Qualitatively analysis, we can find seven kinds of biogenic amines in urines (Put, Oct, 5-HT, E, NE, Dop, Agm).Quantitatively analysis, we can find the different urinary catecholamine amounts of normal people and the patients. Another application in the beers and mastitis milk, we had also found five kinds of biogenic amines in beers (Put, Him, Tyr, PEA, Agm) and one kind of biogenic amines in milk (Put). In the capillary electrophoresis microchip, we could successfully separate five kinds of biogenic amines (Put, Him, 5-HT, Dop, Agm) within 3 minutes. The optimum running buffer was pH 10.1,20 mM Borate/20 mM SDS. You-Zung Hsieh 謝有容 2003 學位論文 ; thesis 115 zh-TW |
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碩士 === 國立交通大學 === 應用化學系 === 91 === Analysis of biogenic amines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence
Student:Yi-Fang Chen Advisor: You-Zung Hsieh
Department of Applied Chemistry
National Chiao Tung University
Abstract
Mono- or polyamines involved in biological activities and that exist in nature are sometimes called by biogenic amines. Some of them are biologically synthesized from amino acids by amino acid decarboxylase degradation and play an important role in living systems. In this study, analysis of fourteen biogenic amines by micellar electrokinetic capillary electrophoresis and laser induce fluorescence were investigated. The selected derivatizing reagent was fluorescein isothiocyanate ( FITC).
In order to achieve an optimum detection sensitivity and separation efficiencies,it was necessary to optimize precolumn derivatization conditions with respect to reaction time, pH, buffer concentration and the molar ratios of FITC to biogenic amines. It must also study the optimum separation conditions. In an optimized condition of micellar electrokinetic chromatography, using 40 mM sodium borate buffer (pH 10.1) with 40 mM sodium dodecyl sufate (SDS), fourteen biogenic amines could be successful separated.
To check the applicability of the method, affective disorder patient’s urine was analyzed. We additional spiked E and NE to identify the existence of E and NE in the urine. Qualitatively analysis, we can find seven kinds of biogenic amines in urines (Put, Oct, 5-HT, E, NE, Dop, Agm).Quantitatively analysis, we can find the different urinary catecholamine amounts of normal people and the patients. Another application in the beers and mastitis milk, we had also found five kinds of biogenic amines in beers (Put, Him, Tyr, PEA, Agm) and one kind of biogenic amines in milk (Put).
In the capillary electrophoresis microchip, we could successfully separate five kinds of biogenic amines (Put, Him, 5-HT, Dop, Agm) within 3 minutes. The optimum running buffer was pH 10.1,20 mM Borate/20 mM SDS.
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author2 |
You-Zung Hsieh |
author_facet |
You-Zung Hsieh Yi-Fang Chen 陳義芳 |
author |
Yi-Fang Chen 陳義芳 |
spellingShingle |
Yi-Fang Chen 陳義芳 nalysis of biogenic amines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence |
author_sort |
Yi-Fang Chen |
title |
nalysis of biogenic amines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence |
title_short |
nalysis of biogenic amines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence |
title_full |
nalysis of biogenic amines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence |
title_fullStr |
nalysis of biogenic amines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence |
title_full_unstemmed |
nalysis of biogenic amines by Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence |
title_sort |
nalysis of biogenic amines by micellar electrokinetic chromatography with laser-induced fluorescence |
publishDate |
2003 |
url |
http://ndltd.ncl.edu.tw/handle/12649392964329096738 |
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