| Summary: | 碩士 === 國立中央大學 === 化學工程與材料工程研究所 === 91 ===
The purpose of this research is to study a porous composite membrane, which can be used in the scaffold of tissue engineering as imitation of extracellular matrix (ECM). The membranes of chitosan/chondroitin sulfate were prepared by polyelectrolytic reaction and freeze-drying methods. In this study different weight ratio of chitosan and chondroitin sulfate were blended and crosslinked with three different crosslinking reagents respectively (glutaraldehyde 、1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS) and calcium ion) to form distinct composite membranes. The basic physical and chemical properties, in vitro assay and the control release behavior of growth factor in the scaffold were studied respectively in this study.
SEM results show that the composite membrane is porous both before and after crosslinking. The aperture of the membranes will decrease after crosslinking and the diameter of aperture distribute between 30 and 200μm. From the weight lost analysis crosslinking will increase the stability of the composites membrane. The stability of the membranes increased 20% after crosslinked by calcium ion. Weather the composite membranes crosslinked or not all shows that the membrane will be degraded in enzyme solution, but the degradation ratio of the crosslinked membrane is lower than that of the uncrosslinked one. Cell toxicity experiments show that uncrosslinked membranes are nontoxic to cell, but the crosslinked membranes were evidenced toxic to cell except the one crosslinked by EDC/NHS. The absorption ability of membranes to basic fibroblast growth factor (b-FGF) weather crosslinked by EDC/NHS, calcium ion or not is higher than 80% while the composite membranes crosslinked by glutaraldehyde for 24 hrs is only 68%. The absorption ability will increase as amine group increased and decrease as carboxyl and sulfinyl groups increased. The controlled released experiments show that the release of b-FGF can be last more than seven days both for crosslinked and uncrosslinked membrane. Weak bonding of b-FGF with carboxyl or sulfinyl groups increased the release amount of b-FGF while strong bonding of b-FGF with amine group decreased the b-FGF amount.
|
|---|
