Functional Characterization of OsGRP3 by gene Overexpression and RNA interference in Rice

• Main Authors: Pei-Ting Chen, 鄭珮婷
• Other Authors: Shin-Lon Ho
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/54839176257231635759

碩士 === 國立嘉義大學 === 農學研究所 === 93 === Abstract A rice glycine-rich protein gene, designated as OsGRP3 (Oryza sativa glycine-rich protein 3), and hase been isolated from rice genome DNA by PCR method. The nucleotide sequence of OsGRP3 is 731 bp, which encodes 186 amino acids. Comparison of the OsGRP3 amino acids sequence with protein databank, we found that the OsGRP3 shares 97 % similarity with rice homologs, OsGRP1. In this study, we conducted the gene-overexoression and gene-silencing strategies to identify the physiological function of OsGRP3. Based on the Agrobacterium-mediated gene transformation, we have obtained 8 independent lines of OsGRP3-overexpressing transgenic plants. PCR amplified of the antibiotic selection marker, hygromycin phosphotransferase gene (Hph), all 8 of the independent transgenic rice showing a 560 bp DNA fragment, indicating that all of them are the OsGRP3 transgenic rice plants.