| Summary: | 碩士 === 國立屏東科技大學 === 食品科學系 === 91 === Abstract
Cancer is the first leading cause of deaths in Taiwan, killing a lot of people every year as well as costing people a large fortune. Therefore, looking for the natural antimutagenicity and anticancer materials becomes the mainstream of biotechnology in the 21st Century. The objective of this study was to evaluate the antimutagenicity of the water-extract and 50% ethanol-extract of the folk medicinal plants Plectranthus amboinicus, Clausena excavata Burme f., Thespesia populnea.
This study was evaluated by Ames Test by using the Salomonella typhimurum TA98, TA102, and TA1535. With direct antimutagenicity agent NQNO(no S9 mix. added) and indirect antimutagenicity agent B[a]P(S9 mix. added), they were to identify the antimutagenicity of the six samples which were from those three plants. The concentrations of the extracts were measured by 1mg/ plate, 1.5mg/ plate, 2mg/ plate, 2.5mg/ plate, and 5mg/ plate. Results obtained in the test clearly demonstrated that Plectranthus amboinicus water-extract and ethanol-extract, either with or without the addition of S9 mix, were toxic toward TA98, TA102, and TA1535 at the concentration of 5mg/ plate. However, with a lower concentration at 2.5mg/ plate, they showed no toxicity. Clausena excavata Burme f. water-extract, either with or without the addition of S9 mix, was toxic toward TA1535 at the concentration of 5mg/ plate. On the contrary, with a lower concentration at 2.5mg/ plate, it showed no toxicity at all. In addition, there was no mutagenicity found among the samples with various concentrations throughout the test.
Plectranthus amboinicus water-extract and Thespesia populnea water- and ethanol- extract showed higher antimutagenicity on TA98 with B[a]P, but Clausena excavata Burme f. ethanol-extract showed the least antimutagenicity. Thespesia populnea ethanol-extract showed higher antimutagenicity on TA102 with B[a]P. On TA1535 with B[a]P, Thespesia populnea water- and ethanol-extract showed higher antimutagenicity, but Plectranthus amboinicus water- and ethanol-extract showed lower antimutagenicity. On TA98 with NQNO, Thespesia populnea water-extract showed higher antimutagenicity. Also, Thespesia populnea water-extract showed higher antimutagenicity on TA102 with NQNO. On TA1535 with NQNO, Clausena excavata Burme f. water and ethanol extract showed higher antimutagenicity. According to these results, Plectranthus amboinicus water-extract had significant antimutagenicity for the mutation induced by TA98, but had no notable antimutagenicity for the mutation induced by TA1535. Plectranthus amboinicus ethanol-extract had significant antimutagenicity for the mutation induced by TA98 with B[a]P, but had no notable antimutagenicity for the mutations induced by TA102 with B[a]P, TA1535 with B[a]P, and TA1535 with NQNO. Clausena excavata Burme f. water-extract had significant antimutagenicity for the mutation induced by TA1535, but had lower antimutagenicity for the mutation induced by TA102 with NQNO. Clausena excavata Burme f. ethanol-extract had significant antimutagenicity for the mutations induced by TA1535 and TA102 with NQNO, but had lower antimutagenicity for the mutation induced by TA102 with B[a]P. Thespesia populnea water-extract had significant antimutagenicity for the mutations induced by TA98 and TA102 with NQNO, but had no notable antimutagenicity for the mutation induced by TA1535 with B[a]P. Thespesia populnea ethanol-extract had significant antimutagenicity for the mutations induced by TA102 and TA102 with B[a]P, but had lower antimutagenicity for the mutation induced by TA1535.
Key words: Plectranthus amboinicus, Clausena excavata，
Thespesia populnea, antimutagenic, Ames Test
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