Comparison between Linear and Nonlinear Estimation of Multifield 15N Relaxation Parameters in Protein.
碩士 === 國立中山大學 === 應用數學系研究所 === 91 === According to the model free approach assumption four protein dynamic related parameters, the slow and fast local motion of the NH vector, the generalized order parameter, and the 15N shielding anisotropy can be estimated at each residue by the spectral density...
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| Format: | Others |
| Language: | en_US |
| Published: |
2003
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| Online Access: | http://ndltd.ncl.edu.tw/handle/57615683606257792153 |
| Summary: | 碩士 === 國立中山大學 === 應用數學系研究所 === 91 === According to the model free approach
assumption four protein dynamic related parameters, the slow
and fast local motion of the NH vector, the generalized order
parameter, and the 15N shielding anisotropy can be
estimated at each residue by the spectral density functions at the
resonant frequencies of N (omega_N) and H (omega_H). In
this work, we study the linear and nonlinear estimations of the
aforementioned parameters of the two proteins C12A-p8^MTCPI
and Pilin from strain K122-4. The principal components of the
four parameters of C12A-p8^MTCPI are used to cluster the residues. The
results show that the principle components provide useful
information about the secondary structure of the protein.
Finally, we propose a practical method to examine the model free
assumption by characterizing the distribution of the transverse
rate R_2 in multifield.
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