Preparation and Application of Fullerene C60 Immobilized Enzyme Electrodes for Glucose and Urea

• Main Authors: Yu-Ying Chang, 張祐穎
• Other Authors: Jeng-Shong Shih
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/89968310831318316439

碩士 === 國立臺灣師範大學 === 化學研究所 === 91 === Abstract Immobilized C60 －Glucose Oxidase and C60-Urease were synthesized and applied in glucose and urea C60-cryptand[2,2] / PVC electrodes to detect glucose and urea in aqueous solutions. The C60-cryptand[2,2] glucose selective membrane electrode detection system to detect the H+ ion which is the product of the gluconic acid dissociation catalyzed by C60-glucose oxidase in another PVC membrane and C60-cryptand[2,2] urea selective membrane electrode detection system to detect the NH4+ which is the production of urea hydrolysis catalyzed by C60-urease in another PVC membrane. The effects of mass of immobilized enzymes, temperature, pH value and interference ions on the potential response were investigated and discussed. For glucose selective membrane electrode detection system, it was found that the catalytic ability of immobilized C60-glucose oxidase showed an optimum temperature at 30℃~35℃ and optimum pH between 6~7, the potential response showed increase with the increased mass of immobilized C60-glucose oxidase in PVC membrane. This electrode gave a linear response 52.3mV/decade within the concentration range in 10-4~10-1 M of glucose and showed selectivity coefficients around 10-2 for most interfering ions, e.g., alkali, alkaline earth and transition metal ions. For urea selective membrane electrode detection system, the optimum is at testing solution pH value 6~7, and optimum temperature at room temperature. This urea electrode also exhibited a linear response 51.7 mV/decade within the concentration range in 10-4~10-1 M of urea and also showed selectivity coefficients around 10-2 for most interfering ions, e.g., alkali, alkaline earth and transition metal ions.