Comparison on the bioactivity of Glycine tomentella Hayata and Flemingia philipinensis Merr ＆ Rolfe and the separation of the bioactive polyphenols in the Glycine root.

• Main Authors: Ying-Yu Kuo, 郭瑩玉
• Other Authors: Bonnie Sun Pan
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/48024351064060838888

碩士 === 國立海洋大學 === 食品科學系 === 91 === Abstract The I-Tiao-Gung, root of Glycine tomentella Hayata has been used extensively as a herbal medicine in Taiwan. It was claimed to have similar functions in analgesic, anti-pyretic, muscle relaxative and blood-circulation enhancing effects to those of the Flemingia philipinensis Merr ＆ Rolfe. However, no extensive research regarding its bioactivities nor its active compounds was found in literature. The objectives of this study were to compare the bioactivities of the roots of the 2 species, and to separate and identify the effective compounds in the Glycine root. The polyphenols content extracted from Glycine, using water, 50 % ethanol and 95 % ethanol as solvents were 4.5, 2, and 1.5 times of the corresponding extract of the Flemingia, respectively. The yield in dry weight of polyphenols of 95 % ethanolic extract of Glycine was higher than that of Flemingia. The two other solvent extracts showed no significant difference between the 2 types of I-Tiao-Gung. Glycine was higher in free radical-scavenging activity determined with DPPH, reduction in hemoglobin-catalyzed lipid autoxidation and inhibition of the lipoxygenase (LOX)-catalyzed arachidonate oxidation. But the inhibition on the cyclooxygenase-catalyzed arachidonate oxidation was higher for the 95 % ethanolic extract of Flemingia than that of Glycine, and was 30,000 times of the positive control, indomethacin. The 95 % ethanolic extract of Glycine was further fractionated using polyamide and silica gel column into 11 fractions. Blood thinning effects were observed in vitro by mixing the Glycine root extracts with human red blood cells. The addition of the 11 fractions of 95 % ethanolic extract of Glycine root increased the flow behavior index (n) from 0.68 to 0.72~0.78, and reduced fluid consistency (K) from 98 to 57~79 cP indicative of a change in flow behavior becoming closer to Newtonian type and a thinning consistency. The antioxidant activities of the 5 fractions eluted from silica gel column were higher than the 95 % ethanolic crude extract. The IC50 values were 15~70 % of the latter. The IC50 values of all fractions on DPPH free radical-scavenging activity were 2~21 times than the 95 % ethanolic crude extracts. The 11 fractions of the 95 % ethanolic extract showed inhibitory ability on LOX being 0.36~1.67 % of the NDGA (nordihydroguaiaretic acid), and 2.6~17.8 times of the 95 % ethanolic extract. The inhibitory activity of the fraction VIII was the highest among the 11 fractions. Fraction VIII was further separated using HPLC, and preliminarily identified using TLC sprayed with coloring agents, and UV-Vis spectrum. Isoflavone without glycoside was considered the bioactive component, which was further identified with NMR to be daidzein and its inhibitory ability on LOX was 1.4 and 23 times of the fraction VIII and the 95 % ethanolic extract.