| Summary: | 碩士 === 國立海洋大學 === 食品科學系 === 91 === Chitosan is a biodegradable, biocompatible, non-toxic polysaccharide. Different forms of chitosan carriers could provide controlled delivery of drug compounds. The objectives of this study were to investigate the preparation of coacervated and cross-linked chitosan (with a degree of deacetylation of 81% and an average molecular weight of 70 kDa)─pectin particles, and to measure the release behavior of amoxicillin.
The average diameter of air-dried chitosan-pectin particles is smaller than that of the freeze-dried samples and their appearance is darker than freeze-dried particles. The stability of amoxicillin at pH 7.4 was better than at pH 1.4. The degradation of amoxicillin obeyed first-order kinetics.
In pH 1.4 environment, genipin and glutaraldehye crosslinked chitosan-pectin particles slowly dissolved. In pH 7.4 environment, genipin and glutaraldehye crosslinked chitosan-pectin particles swelled less. All chitosan-pectin particles swelled, water diffused in, then they collapsed and dissolved.
The drug loaing of chitosan-pectin particles, genipin crosslinked, glutaraldehye crosslinked and TPP crosslinked particles was 61%, 57%, 51% and 49% respectively.
The release of amoxicillin in pH 1.4 buffer was lower than 6%. The release of amoxicillin in pH 7.4 buffer after 720 min from TPP crosslinked, chitosan-pectin, glutaraldehye crosslinked and genipin crosslinked particles was 65% ,57%, 19% and 14% respectively. The release of amoxicillin in pH 7.4 buffer (in the presence of pectinase) after 720 min from TPP crosslinked, chitosan-pectin, glutaraldehye crosslinked and genipin crosslinked particles was 93% ,90%, 69% and 21% respectively.
The characteristics of crosslinked particles were different because of the difference in crosslinking mechanism. Coacervated and TPP crosslinked chitosan-pectin particles released amoxicillin faster. In contrast, genipin and glutaraldehye crosslinked particles could provide extended release of amoxicillin. Genipin may be a good alternative crosslinking agent for glutaraldehye.
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