Effects of the Sand Particle Size upon Glomus spinosum Spores Production and Semi-Aseptic Synthesis of Tuber pseudoexcavatum

• Main Authors: Jia-Hou Chen, 陳珈后
• Other Authors: Hung-Tao Hu
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/94645320253354646001

碩士 === 國立臺灣大學 === 森林學研究所 === 91 === purposes of this study were to（1）compare the spores production of Glomus spinosum cultured with Cunninghamia lanceolata in different sand particle sizes and（2）perform semi-aseptic synthesis of Tuber pseudoexcavatum on Cyclobalanopsis glauca（Thunb.）Qerst. From the results of experiment 1：（1）it was shown that the amounts of Mg＋2、 Ca＋2 and SO4－2 in substrate were significantly different（P＜0.05）among different sand particle sizes. In terms of Mg＋2 amounts in sand, the order was sand particle size 150-50 µm＞greater than 420 µm＝210-150 µm＝420-210 µm. In terms of Ca＋2 amounts in sand, the order was sand particle size 150-50 µm＞greater than 420 µm＝210-150 µm＞420-210 µm. In terms of SO4－2 amounts in sand, the order was sand particle size 150-50 µm＞210-150 µm＝420-210 µm＝greater than 420 µm. Therefore, the amounts of Mg＋2、Ca＋2 and SO4－2 were the most in the cultured sand particle size 150-50 µm. （2）Spore amounts attached at root and produced in different particle sizes of sand were significantly different（P＜0.05）among those sand particle sizes. In terms of spore amounts attached at root, the order was those cultured in 420-210 µm＝those cultured in greater than 420 µm＞those cultured in 210-150 µm＞those cultured in 150-50 µm. In terms of spore amounts produced in different particle sizes, the order was those cultured in 420-210 µm＞those cultured in greater than 420 µm＝those cultured in 210-150 µm＝those cultured in 150-50 µm. Therefore, sand particle size between 420-210 µm was best for spores production compared with those cultured in other sand particle sizes treatment. Spore amounts attached at root and measured by root fragment showed significant relationship with root dry weight（r＝0.98；r＝0.96）respectively. The spore amounts produced in sand culture showed significant positive relationship with seedlings top dry weight（r＝0.97）. From the results of experiment 2, the characteristics of ectomycorrhizae formed by Tuber pseudoexcavatum and Cyclobalanopsis glauca were summarized as follow: monopodial, with smooth surface, with or without bristles; ectomycorrhiza was light yellowish-brown when it was young and turned to brown and dark brown, when it became mature and old. The structure of outer mantle was with felt prosenchyma, and that of inner mantle was with regular synenchymatic pseudosynenchyma; Hartig nets could extend to the third layer of the cortex cells.