Effects of Nucleosides and Nucleotides Mixture, Vitamin E and Tetrahydrobiopterin on Learning and Memory Ability and Antioxidative Status in Senescence Accelerated Mice.

• Main Authors: Pei-Hsin Chen, 陳沛欣
• Other Authors: Ming-Fu Wang
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/ark5g6

碩士 === 靜宜大學 === 食品營養研究所 === 91 === The aging process accompanies pathological changes of brain and retardation of physiological function. The purpose of this study was to investigate the effects of 0.5% nucleosides-nucleotides mixture (NNM), 0.01% vitamin E (Vit. E) or 0.001% tetrahydrobiopterin (BH4) supplementation on learning and memory ability and antioxidative status in senescence-accelerated mouse prone-8 (SAMP8). Seven month-old SAMP8 used as subjects were divided into control group and experimental groups which were supplemented with NNM, Vit. E or BH4 for twelve weeks. Body weight, food intake, grading score, open field activity test, active shuttle avoidance test and single-trial passive avoidance test were performed during the experiment. After animals sacrificed, this experiment analyzed the biochemical parameters, total antioxidant states and vitamin E concentration of blood, weighed tissue weights, tested in superoxide dimutase activity on liver and brain of tissue and observed the brain histopathology of lipofuscin and spongy degeneration. According to the results, body weight, food intake, open field activity test, blood biochemical parameters and tissue weight all had no significant difference between four groups. Vitamin E and NNM supplementation could improve the aging condition that deteriorated in grading score, active shuttle avoidance test, vitamin E concentration and brain histopathology (p＜0.05). The improving effects of total antioxidant states, superoxide dimutase activity on tissues and vitamin E concentration were the most significant on Vit. E group (p＜0.05). Furthermore, the antioxidative ability also was had enhanced tendency by the supply of NNM. But the BH4 supplement diet had less improvement for learn and memory ability and antioxidative defense ability and brain physiology. Therefore, the diets of supplementation NNM and Vit. E had the improving effect to delay the proceeding of the aging process, and oral supplement BH4 had less improving effect.