| Summary: | 碩士 === 東海大學 === 化學系 === 91 === The XPRK motif as a novel DNA binding motif is studied by our laboratory in recent years. This thesis reports on the solid-phase synthesis of three series of peptides containing the XPRK motif：
1.Five linear peptides containing repeating XPRK motifs.
2.Eight linear peptides containing N-methylpyrrole residues(Py).
3.Seventeen cyclic peptides containing repeating XPRK motifs.
Agarose gel electrophoresis showed that peptides containing Py residues have some DNA nicking activities. Similarly, if the Pro residues of the peptide is replacedwith Hyp residues, the DNA nicking activities of the peptides are increased. A number of cyclic peptides synthesized possess good DNA binding activities, and gel retardation studies by agarose gel electrophoresis showed that the 14-mer cyclic peptides possess better DNA binding activities than the 10-mer cyclic peptides.Footprining studies by Lin Pei-Hsuan showed that the cyclic peptides CRHY-14, CH2Y-14, CHyD-14, CHyY-14 and CMY-14 possess DNA sequence selective binding, with major selectivity on 5''-TATTT-3'', 5''-TTTTCT-3'' and 5''-TATTT-3'' sequences.
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