| Summary: | 碩士 === 臺北醫學大學 === 口腔復健醫學研究所 === 91 === Abstract
This in vivo study was to examine the historical changes of implanted novel chitosan/collagen composite barrier for confirming the clinical feasibility. Four other commercial GTR (Guided Tissue Regeneration) membranes were chosen for comparison. Among the resorbable GTR membranes, BioMend Extend™ and Peri-Aid® are collagen base, and GORE-TEX® OSSEOQUEST is synthesized membrane, while GORE-TEX® e-PTFE (Expanded polytetrafluoroethylene) is synthesized but non-resorbable. Beagle dogs were used as animal model. Buccal mucoperiosteal flaps were reflected in the bilateral mandibular premolar and molar areas. Buccal alveolar bone was reduced on 1st、2nd premolar and molar to a level 5 mm apical to the cemento-enemel junction (CEJ). Root surface was denuded of periodontal ligament and cementum, and notches were placed at the bone level of each root. The tested GTR barriers were implanted in critical bone defect areas. Flaps were coronally positioned and sutured. Two beagle dogs were sacrificed each time as the designed time period after surgery. Histological and histometirc evaluation at 7 days、14 days、28 days、3 months were performed post-operatively to determine the healing response of each treatment modality. Both the cementum height and bone height were measured as the index of tissue occlusion effect. Like all resorbable GTR membrane, the chitosan/collagen composite barrier enhanced the cementum regeneration of 1.16 mm averagely after 28-day implantation. After 3 months, average cementum height of 2.6 mm was observed for chitosan/collagen composite barrier group. On the contrast, cementum height of 0.9 mm was observed in e-PTFE group. Meanwhile bone regeneration also observed and bone height was measured average 1.0 mm for all test membrane group except the control group without GTR membrane implanted reveal no bone formation. In our study, inhibiting epithelial migration and encouraging formation of new connective tissue attachment to root surface evidenced positive results of chitosan/collagen composite barrier placement. It also promoted blood clot aggregation and maturation in early wound healing process and decreased wound infection.
Key word：chitosan, collagen, guided tissue regeneration, cementum height, alveolar bone regeneration.
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