| Summary: | 碩士 === 元智大學 === 化學工程學系 === 91 === Abstract
This research studied the effects of the surface micropatterns and properties of biomaterials on the cell’s activities for the application in tissue engineering. It is expected to develop a fast, safe, and cost effective fabrication process for making membranes with surface micropatterns. Masters with micropattern spacing in 200μm were prepared by photolithography. The membranes with surface micropattern were then fabricated by soft lithography and dry-wet phase inversion techniques. The concentration and composition of the polymers and the operating temperature can be adjusted to optimize the process. Chitosan, collagen, and gelatin blended membranes with micropatterns were prepared for cell culture. C3A cells showed activities of cell growth, adhesion, communication, and migration on the membranes. The membranes were intact without evident degradation during cell culture after 14 days. On the membranes with micropattern spacing of 200μm in width and 80μm in depth, C3A cells aggregated on the grooves, and showed the higher growth and metabolism rate; the C3A cells on the ridge were sparse and had long pseudopodium in morphology. However, the phenomena were not found on the membranes with micropattern spacing of 500μm in width. It indicated that micropattern on the membrane surface could affect the communication among cells and result in difference of the properties of adhesion, morphology, and mobility of the cells.
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