| Summary: | 碩士 === 長庚大學 === 化工與材料工程研究所 === 92 === The purpose of this study is to produce human serum albumin by transgenic rice callus. For the past two decades, many scientists used biotechnology to produce biopharmaceutical proteins in transgenic plants. Using plants as expression system for producing biopharmaceutical proteins from human or animals may avoid pollution of unknown viruses’ contamination by traditional extraction methods.
In order to enlarge expression of recombinant human serum albumin from the αamy3 promoter in the rice Tainung 67（TNG67）, we carried out suspension culture and immobilization culture to produce human serum albumin. In manufacture, the amount of human serum albumin by suspension culture was larger than by immobilization culture. However, immobilization can protect cell from harm of shear force, and with better cell viability. On the part of immobilization by flask, most calli was immobilizated in the upper section of support. In order to amplify making use of support, we carried out two-way bubble cycle reactor to make calli distribute uniform.
Make a comprehensive survey on the result between suspension culture and immobilization culture. Immobilization culture not only can reuse cell but also benefited continue manufacture.
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