| Summary: | 碩士 === 中國醫藥大學 === 中國藥學研究所 === 92 === This present study was aimed to investigate the ameliorating effects and its mechanism of Flos Lonicerae and its constituent-luteolin on scop0lamine- induced memory impairment in rats. First, we evaluate the ameliorating effects of Flos Lonicerae on scopolamine-induced impairment of passive avoidance response in rats. Flos Lonicerae crude extracts ameliorated scopolamine-induced impairment of passive avoidance response. Then ethyl acetate layer among four layers of Flos Lonicerae has better potency.
Secondly, we clarified the relationship of central and peripheral nervous system on the ameliorating effect of ethyl acetate layer of Flors Lonicerae in rats. The data found peripheral cholinergic antagonist-scopolamine methybromide did not blocked the reversal from scop-induced impairment, but ethyl acetate layer of Flos Lonicerae reversed the impairment of passive avoidance response, spatial performance and intermediate reference memory induced by central cholinergic neurotoxin Ethylcholine aziridinium (AF64A) in rats. Finally, we demostrated the role of central nervous system on the ameliorating effect of luteolin-a constituent of Flos Lonicerae by using central neurotoxin and central nervous antagonists . The data point out luteolin ameliorated the impairment of passive avoidance response and deficit of memory process.
From these above verdicts, we suggested Flos Lonicerae posseses ameliorating activity for memory deficit, and its main constituents exist in ethyl acetate layer. Luteolin, a constituent of Flos Lonicerae, also ameliorated the memory deficit. The action mechanism of luteolin might be activated the central cholinergic catecholamine system, and mainly relate to activate muscarinic and muscarinic receptors.
|
|---|
