Pharmacological studies of Ampelopsis cantoniensis An approach on antinociceptor, anti-inflammation, N-acetyltransferase and HL-60 Cell Line

• Main Authors: Tzu-Wei Tan, 譚思濰
• Other Authors: 蔡輝彥
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/66574641474992120547

博士 === 中國醫藥大學 === 中國藥學研究所 === 99 === Pharmacological studies of Ampelopsis cantoniensis An approach on antinociceptor, anti-inflammation, N-acetyltransferase and HL-60 Cell Line Tzu-Wei Tan Institute of Chinese Pharmaceutical Sciences China Medical University Ampelopsis cantoniensis (Hook. et Arn.) Planch. is a plant of Ampelopsis of Vitaceae. It grows in bushes or luxuriant forests mainly in low altitude zone of mountains throughout Taiwan. It has been often used as anti-inflammatory and analgesic drug in folk medicine, but there are few reports to address its pharmacological mechanisms. The crude extract of Ampelopsis cantoniensis was partitioned with various solvents into n-hexane layer, chloroform layer, n-butanol layer and water layer. We attempted to explore the effect of Ampelopsis cantoniensis on its pharmacological properties. In the formalin test, the crude extract and partitioned extract of Ampelopsis cantoniensis could significantly inhibited the licking response both on the central and peripheral response. It might be related to NMDA receptor. In the anti-inflammatory experiments, the crude extract and partitioned extract of Ampelopsis cantoniensis inhibited the release of inflammatory substances. The partitioned extract from larger polar layers (n-butanol and water layers) had much more significant inhibition (both on the formalin test and on anti-inflammatory experiment) than that of other layers. The crude extract and partitioned extract of Ampelopsis cantoniensis significantly inhibited the activation of acetyltransferase both on the liver tissue and blood cytosols of rats. In vivo experiments, Ampelopsis cantoniensis inhibited the acetylation of arylamines by administration either pretreated or post-treated with arylamines. The crude extract of Ampelopsis cantoniensis arrested the G0/G1 phase of cell cycle on HL-60 cells. It inhibited the gene expression of cyclin E, cyclin D1, cyclin D2 and cyclin D3 on cell cycle HL-60. Based on the results from PCR and cDNA microarray, the cell apoptosis of HL-60 might be attributed to the activation of cytochrome C and cytochrome C1 in mitochondria, caspase 9, followed by activating caspase 3 after treatment with the crude extract of Ampelopsis cantoniensis.