The study in the relationship between effect of Carthamus tinctorious L. on Ischemia-Reperfusion cerebral infarct Rats, and tumor necrosis factor-α and interleukin-1β

• Main Authors: Fu, Pin-Kuei, 傅彬貴
• Other Authors: Hsieh, Ching-Liang
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/08753122800013395420

碩士 === 中國醫藥大學 === 中國醫學研究所 === 99 === According to the theory of Traditional Chinese medicine, cerebrovascular accident mainly results from blood stasis. Carthamus tinctorious L. (CT) is considered that has the action of activate blood to eliminate stasis since long time ago. Several studies have known recently that CT has antioxidant, ant inflammation and inhibiting glutamate-mediated injury action, and also can protect neuronal cell in the brain. Therefore, the purpose of the present study is to investigate effect of CT on cerebral infarct. A total of 72 male Sprague-Dawley (SD) rats were studied. In experiment one (54 SD), an animal model of cerebral infarct was established by occluding bilateral common carotid arteries (CA) and the right middle cerebral artery (MCA) for 90 min, then reperfusion for 24 hrs. Intra- peritoneal (ip) administration of CT 0.2 g/kg, 0.4 g/kg, 0.6 g/kg and MK801 1.0 mg/kg 10 min before , and CT 0.4 g/kg 30 min after occluding the cerebral blood flow, respectively. In addition, CT 0.4 g/kg i.p. was done 30 min after reperfusion of 2 hrs. The cerebral infarct size and grade of neurological deficit were used as an index to evaluate the effect of CT on cerebral infarct. The superoxide anion was measured by lucigenin- Chemiluminescence (CL) counts before and 90 min after occluding the cerebral blood flow, and 2 hrs after reperfusion, respectively. The tumor necrosis factor-alfa (TNF-α) and interleukin-1beta (IL-1β) immunostaining in the core of cerebral infarct area, and the levels of blood sugar were also measured 24 hrs after reperfusion. The results indicated that pretreatment with CT 0.2 g/kg, 0.4 g/kg, 0.6 g/kg, MK801, and post-treatment with CT 0.4 g/kg all decreased the ratio of cerebral infarct area. Pretreatment with CT 0.4 g/kg or 0.6 g/kg also decreased the grade of neurological deficit. Pretreatment with CT 0.4 g/kg can decrease the lucigenin-CL counts at reperfusion of 2hrs, and it also can decreased the counts of both TNF-α and IL-1β immunostaining positive cells in the cerebral infarct area, but the levels of blood sugar and rectal temperature were similar to the control. In conclusion, CT can decrease both the ratio of cerebral infarct area and grade of neurological deficit, suggesting can be used to treat acute stage of cerebral infarct in humans. This effect of CT has relationship to inhibit superoxide anion to reduce the generation of oxygen free radicals, and decreased proinflammatory cytokine TNF-a and IL-1β resulting to inhibit inflammatory response, but no relationship to blood sugar and rectal temperature were noted. Keywords: Carthamus tinctorious L., Cerebral infarct, Neurological deficit, Superoxide anion, TNF-α、IL-1β