Prevalence of Antimicrobial Resistance in Proteus mirabilis in Middle Taiwan Hospitals

• Main Authors: WU HUA JEN, 吳華真
• Other Authors: 吳禮字
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/16736056172840917172

碩士 === 中國醫藥大學 === 醫學研究所 === 92 === ABSTRACT Strains of Proteus mirabilis were isolated from patient sufferings by respiratory tract and urinary tract infection during February and June 2003 from middle Taiwan hospitals. These strains were resistant to ampicillin, gentamicin, amikacin and cefotaxime, while they were highly susceptible to ceftazidime (MIC, ≦0.5 mg/ml) and meropenem(MIC, <0.5 mg/ml) were characterized. Out of a total 111 clinical isolates 34 (30.6%) strains exhibited the resistance level to cefotaxime was decreased by the presence of clavulanic acid. Therefore, these strains were speculated to produce extended-spectrum class A b-lactamases. By isoelectric point determination, PCR, and nucleotide sequencing, we detected the presence of CTX-M-3, and TEM-1in 1 strain, CTX-M-3, CTX-M-14, and TEM-1in 5 strains, CTX-M-14 and TEM-1 in 19 strains and CTX-M-14 only in 9 strains. This multiple drug resistance genes were shown to be transferred by a conjugative plasmid. The 34 Proteus mirabilis strains belong to 9 different genotypes were classified by SfiI Pulse Field Gel Electrophoresis (PFGE) patterns. Base on PCR, the sequence of the class I integron with gene cassette in 33 strains and tnpA transposase gene of the insert sequence ISEcp1 in some strains were also detected. In this study, the presence of Proteus mirabilis strains with high percentage CTX-M type of extended-spectrum b-lactamases (ESBLs) was first demonstrated in Taiwan.