Ellagic acid inhibit vascular smooth muscle cell proliferation and apoptosis induced by ox-LDL

• Main Author: 曾瓊瑤
• Other Authors: 張文正
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/69140210835997007779

碩士 === 中國醫藥大學 === 醫學研究所 === 92 === 英文摘要 In the multifunctional pathogens of atherosclerosis, oxidatively modified low density lipoprotein plays a central role in atherogenesis. Previous studies demonstrated that both native LDL and ox-LDL could stimulate the proliferation of vascular smooth muscle cells. However ox-LDL has also been shown to induce apoptosis in vascular smooth muscle cells (VSMCs). Ellagic acid, an antioxidant, is a phenolic compound presenting in fruits (raspberries, blueberries, strawberries) and walnuts. In this study, we investigated whether VSMCs proliferation and apoptosis induced by ox-LDL would be affected by ellagic acid. Cell viability was tested by MTT test and cell count and study ox-LDL stimulated cell proliferation was inhibited by Ellagic acid. Ellagic acid suppressed the increased the percetage of cell cycle S phase induced by ox-LDL. The activitives of ErK 1/2 , pErK 1/2 and PCNA were also inhibited by ellagic acid. In apoptosis, ellagic acid also inhibited cell apoptosis and cell morphologic change. However ellagic acid suppressed expression of caspase genes could by ox-LDL. These results might significantly advance the understanding of the antioxidants implicated in the formation of atherosclerosis, and could eventually lead to the development of new dietary supplements for the prevention of atherosclerosis. Key word: Ellagic acid, Ox-LDL, Smooth muscle cell, Proliferation, Apoptosis.