Effect of 3,6-subsituted 9- pyridylmethylcarbazole derivatives on differentiation of HL-60 cells

• Main Authors: Feng Chun-Ta, 馮鈞達
• Other Authors: 郭盛助
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/01323849219618231430

碩士 === 中國醫藥大學 === 藥物化學研究所 === 92 === In order to develop the novel leukemia cell differentiation agents, a series of 3,6-subsituted 9-pyridylmethylcarbazole derivatives was been screened for their induced differentiation and growth inhibition activities of HL-60 cells. According to the results, when using compound HYL-3b along, it was the most effective to differentiation compared with the other compounds. And it is shown the great coefficient effect to differentiation when combined with all-trans retinoic acid (ATRA). Therefore, the further studies of action mechanism were carried out to evaluate its effects on the cell cycle of HL-60 cells. When treatment of compound HYL-3b alone, it was found to affect the cell cycle as the same way of ATRA, which induced G0/ G1 phase arrest of HL-60 cells. This finding suggests that compound HYL-3b maybe has great potential in the treatment of acute promyelocytic leukemia (APL) either dose alone or together with ATRA.