| Summary: | 碩士 === 中國醫藥大學 === 中西醫結合研究所 === 92 === The marketing of medical cosmetics, especially for treating pigmented problems and mainly melasma is rapidly developing and has an unlimited potential in the current trend. Hydroquinone used to be used for bleaching with number of side effects been reported. Therefore, the studies on the Chinese herbs or other nature products for inhibiting melanogenesis are the tendency of researching this time. The purpose of this study is to assay the bleaching efficiencies of the extract of Sophora japonica. In our studies, distilled water, 50% alcohol and 95% alcohol were used as solvents to extract the dried Sophora flower buds. One half of each extract samples were hydrolyzed with sulfuric acid. The tyrosinase inhibiting efficiencies were evaluated by comparison the efficacies between extracted samples and commercial available bleaching agents (i.e. ascorbic acid, BHA and arbutin). The sophora extract by distilled water with further hydrolysis was found to be the most effective one based on its influences on the WS1 and RPMI 7951 cell culture studies. The results revealed that sophora extract didn’t disturb the cell growth in WS1 cell culture lines, but could inhibit cell growth with a dose-related effect in RPMI7951 cell culture lines. Herein, it was concluded that the Sophora japonica extracted with distilled water with further hydrolysis is a safe and an effective bleaching agent. However, the detailed mechanism should be merit to investigate.
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