Study the biological function of ZAK interacting with 14-3-3 proteins
碩士 === 中山醫學大學 === 生物化學研究所 === 93 === 14-3-3 proteins expressed abundantly in human’s brain. There are seven isoforms of 14-3-3 in mammalian. Each isoform regulates different cellular functions. 14-3-3 proteins could modulate many cellular processes that are involved in various cellular si...
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ndltd-TW-092CSMU01070092016-01-04T04:08:50Z http://ndltd.ncl.edu.tw/handle/88681834969463685761 Study the biological function of ZAK interacting with 14-3-3 proteins 探討ZAK磷酸激酶與14-3-3蛋白結合之生物功能 Shih-Llung Chang 張世龍 碩士 中山醫學大學 生物化學研究所 93 14-3-3 proteins expressed abundantly in human’s brain. There are seven isoforms of 14-3-3 in mammalian. Each isoform regulates different cellular functions. 14-3-3 proteins could modulate many cellular processes that are involved in various cellular signal pathways including cell cycle progression, transcriptional control and apoptosis. Numbers of 14-3-3 family also interact with proteins belong to the Mitogen-activated protein kinase (MAPK) pathway such as PKC, Raf, ASK1, MEKK1, MEKK2 or MEKK3 and regulate cellular differentiation and apoptosis. ZAK (leucine-zipper and sterile-α motif kinase) protein is a novel MAP3K protein kinase. Reports had been demonstrated that ZAK protein could activate JNK and induce cell apoptosis. In this study, we have investigated whether 14-3-3 γ protein associates with ZAK protein and regulates ZAK-induced cell apoptosis. We have performed GST-pull down, western blot and flow cytomertry approaches to determine the biological functions and interactions between 14-3-3 γ and ZAK. We have found the interaction of ZAK protein and 14-3-3 γ was through residues 340 to 460 of ZAK and residues 1 to 159 of 14-3-3 γ. However, the interaction of ZAK with 14-3-3 γ neither changes the ZAK-induced apoptosis nor alters the subcellular localization of ZAK. Li-Sung Hsu 許立松 2004 學位論文 ; thesis 94 zh-TW |
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碩士 === 中山醫學大學 === 生物化學研究所 === 93 === 14-3-3 proteins expressed abundantly in human’s brain. There are seven isoforms of 14-3-3 in mammalian. Each isoform regulates different cellular functions. 14-3-3 proteins could modulate many cellular processes that are involved in various cellular signal pathways including cell cycle progression, transcriptional control and apoptosis. Numbers of 14-3-3 family also interact with proteins belong to the Mitogen-activated protein kinase (MAPK) pathway such as PKC, Raf, ASK1, MEKK1, MEKK2 or MEKK3 and regulate cellular differentiation and apoptosis. ZAK (leucine-zipper and sterile-α motif kinase) protein is a novel MAP3K protein kinase. Reports had been demonstrated that ZAK protein could activate JNK and induce cell apoptosis. In this study, we have investigated whether 14-3-3 γ protein associates with ZAK protein and regulates ZAK-induced cell apoptosis. We have performed GST-pull down, western blot and flow cytomertry approaches to determine the biological functions and interactions between 14-3-3 γ and ZAK. We have found the interaction of ZAK protein and 14-3-3 γ was through residues 340 to 460 of ZAK and residues 1 to 159 of 14-3-3 γ. However, the interaction of ZAK with 14-3-3 γ neither changes the ZAK-induced apoptosis nor alters the subcellular localization of ZAK.
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author2 |
Li-Sung Hsu |
author_facet |
Li-Sung Hsu Shih-Llung Chang 張世龍 |
author |
Shih-Llung Chang 張世龍 |
spellingShingle |
Shih-Llung Chang 張世龍 Study the biological function of ZAK interacting with 14-3-3 proteins |
author_sort |
Shih-Llung Chang |
title |
Study the biological function of ZAK interacting with 14-3-3 proteins |
title_short |
Study the biological function of ZAK interacting with 14-3-3 proteins |
title_full |
Study the biological function of ZAK interacting with 14-3-3 proteins |
title_fullStr |
Study the biological function of ZAK interacting with 14-3-3 proteins |
title_full_unstemmed |
Study the biological function of ZAK interacting with 14-3-3 proteins |
title_sort |
study the biological function of zak interacting with 14-3-3 proteins |
publishDate |
2004 |
url |
http://ndltd.ncl.edu.tw/handle/88681834969463685761 |
work_keys_str_mv |
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