Study the biological function of ZAK interacting with 14-3-3 proteins

• Main Authors: Shih-Llung Chang, 張世龍
• Other Authors: Li-Sung Hsu
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/88681834969463685761

碩士 === 中山醫學大學 === 生物化學研究所 === 93 === 14-3-3 proteins expressed abundantly in human’s brain. There are seven isoforms of 14-3-3 in mammalian. Each isoform regulates different cellular functions. 14-3-3 proteins could modulate many cellular processes that are involved in various cellular signal pathways including cell cycle progression, transcriptional control and apoptosis. Numbers of 14-3-3 family also interact with proteins belong to the Mitogen-activated protein kinase (MAPK) pathway such as PKC, Raf, ASK1, MEKK1, MEKK2 or MEKK3 and regulate cellular differentiation and apoptosis. ZAK (leucine-zipper and sterile-α motif kinase) protein is a novel MAP3K protein kinase. Reports had been demonstrated that ZAK protein could activate JNK and induce cell apoptosis. In this study, we have investigated whether 14-3-3 γ protein associates with ZAK protein and regulates ZAK-induced cell apoptosis. We have performed GST-pull down, western blot and flow cytomertry approaches to determine the biological functions and interactions between 14-3-3 γ and ZAK. We have found the interaction of ZAK protein and 14-3-3 γ was through residues 340 to 460 of ZAK and residues 1 to 159 of 14-3-3 γ. However, the interaction of ZAK with 14-3-3 γ neither changes the ZAK-induced apoptosis nor alters the subcellular localization of ZAK.