Protective Effect of Antioxidants on MPTP- and Rotenone-Induced Neurotoxicity in Neuroblastoma SH-SY5Y cells
碩士 === 中山醫學大學 === 生物化學研究所 === 92 === Recently, it have been shown that rotenone and MPTP, a specific inhibitor of mitochondrial complexⅠ, is a useful tool in animal model of Parkinson’s disease. In this thesis, a human dopaminergic neuronblastoma SH-SY5Y cell line was used to study the effect of exp...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2004
|
Online Access: | http://ndltd.ncl.edu.tw/handle/89509397275698027261 |
id |
ndltd-TW-092CSMU0107015 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-092CSMU01070152016-01-04T04:08:50Z http://ndltd.ncl.edu.tw/handle/89509397275698027261 Protective Effect of Antioxidants on MPTP- and Rotenone-Induced Neurotoxicity in Neuroblastoma SH-SY5Y cells 抗氧化物對神經細胞遭受神經毒害之保護作用研究 Jia-Shiong Chen 陳嘉雄 碩士 中山醫學大學 生物化學研究所 92 Recently, it have been shown that rotenone and MPTP, a specific inhibitor of mitochondrial complexⅠ, is a useful tool in animal model of Parkinson’s disease. In this thesis, a human dopaminergic neuronblastoma SH-SY5Y cell line was used to study the effect of exposure to rotenone and MPTP on cell death, activation of stress-induce pathway and aggregation ofα-synuclein, the characteristic protein accumulated in lewy bodies and mimic the pathogenesis of Parkinson’s disease. Moreover it was investigated that the role of antioxidants play in the rotenone- and MPTP-induced α-synuclein aggregattion, oxidative stress and apoptosis. The results showed that both toxins induce a time and dose-dependant decrease in cell survival with IC50 approached values of 0.5 μM after 48hr for rotenone and 1 mM after 24h for MPTP, respectively. Rotenone and MPTP induced apoptosis was demonstrated by using trypan blue exclusion assay, PI staining, DNA fragmentation, Western blotting assay. In addition, it was found that rotenone and MPTP induced apoptosis an increase in ROS production. The Rotenone- and MPTP-induced cell death and caspase 3 activation were reduced by pretreatment with antioxidants (N-acetyl-cystein (10 mM), Baicalein(40 μM), Acetyl-L-Carnitine(0.5 mM) and Lipoic acid (0.1 mM)),indicated a role of ROS in the neurotoxicity. These results suggested that the antioxidants prevent neurons cells from rotenone and MPTP induce apoptosis through decreasing oxidative stress. Li-Sung Hsu, Ph. D. 許立松 2004 學位論文 ; thesis 109 zh-TW |
collection |
NDLTD |
language |
zh-TW |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 中山醫學大學 === 生物化學研究所 === 92 === Recently, it have been shown that rotenone and MPTP, a specific inhibitor of mitochondrial complexⅠ, is a useful tool in animal model of Parkinson’s disease. In this thesis, a human dopaminergic neuronblastoma SH-SY5Y cell line was used to study the effect of exposure to rotenone and MPTP on cell death, activation of stress-induce pathway and aggregation ofα-synuclein, the characteristic protein accumulated in lewy bodies and mimic the pathogenesis of Parkinson’s disease. Moreover it was investigated that the role of antioxidants play in the rotenone- and MPTP-induced α-synuclein aggregattion, oxidative stress and apoptosis. The results showed that both toxins induce a time and dose-dependant decrease in cell survival with IC50 approached values of 0.5 μM after 48hr for rotenone and 1 mM after 24h for MPTP, respectively. Rotenone and MPTP induced apoptosis was demonstrated by using trypan blue exclusion assay, PI staining, DNA fragmentation, Western blotting assay. In addition, it was found that rotenone and MPTP induced apoptosis an increase in ROS production. The Rotenone- and MPTP-induced cell death and caspase 3 activation were reduced by pretreatment with antioxidants (N-acetyl-cystein (10 mM), Baicalein(40 μM), Acetyl-L-Carnitine(0.5 mM) and Lipoic acid (0.1 mM)),indicated a role of ROS in the neurotoxicity. These results suggested that the antioxidants prevent neurons cells from rotenone and MPTP induce apoptosis through decreasing oxidative stress.
|
author2 |
Li-Sung Hsu, Ph. D. |
author_facet |
Li-Sung Hsu, Ph. D. Jia-Shiong Chen 陳嘉雄 |
author |
Jia-Shiong Chen 陳嘉雄 |
spellingShingle |
Jia-Shiong Chen 陳嘉雄 Protective Effect of Antioxidants on MPTP- and Rotenone-Induced Neurotoxicity in Neuroblastoma SH-SY5Y cells |
author_sort |
Jia-Shiong Chen |
title |
Protective Effect of Antioxidants on MPTP- and Rotenone-Induced Neurotoxicity in Neuroblastoma SH-SY5Y cells |
title_short |
Protective Effect of Antioxidants on MPTP- and Rotenone-Induced Neurotoxicity in Neuroblastoma SH-SY5Y cells |
title_full |
Protective Effect of Antioxidants on MPTP- and Rotenone-Induced Neurotoxicity in Neuroblastoma SH-SY5Y cells |
title_fullStr |
Protective Effect of Antioxidants on MPTP- and Rotenone-Induced Neurotoxicity in Neuroblastoma SH-SY5Y cells |
title_full_unstemmed |
Protective Effect of Antioxidants on MPTP- and Rotenone-Induced Neurotoxicity in Neuroblastoma SH-SY5Y cells |
title_sort |
protective effect of antioxidants on mptp- and rotenone-induced neurotoxicity in neuroblastoma sh-sy5y cells |
publishDate |
2004 |
url |
http://ndltd.ncl.edu.tw/handle/89509397275698027261 |
work_keys_str_mv |
AT jiashiongchen protectiveeffectofantioxidantsonmptpandrotenoneinducedneurotoxicityinneuroblastomashsy5ycells AT chénjiāxióng protectiveeffectofantioxidantsonmptpandrotenoneinducedneurotoxicityinneuroblastomashsy5ycells AT jiashiongchen kàngyǎnghuàwùduìshénjīngxìbāozāoshòushénjīngdúhàizhībǎohùzuòyòngyánjiū AT chénjiāxióng kàngyǎnghuàwùduìshénjīngxìbāozāoshòushénjīngdúhàizhībǎohùzuòyòngyánjiū |
_version_ |
1718159065043959808 |