| Summary: | 碩士 === 中山醫學大學 === 免疫學研究所 === 92 === Abstract
OBJECTIVE： Serologic test currently available for use in Lyme disease. Using different antigen preparation methods extract Borrelia burgdorferi strain B31 specific antigen to study the relationship between Lyme disease, arthritis and other autoimmune diseases in serologic diagnosis. To investigate the seroreactivity to B. burgdorferi strain B31 and B. burgdorferi strain 2591antigens of patients suffering from Lyme disease, arthritis and other autoimmune disease.
METHODS：Antigen preparations for the tests include Lysis Buffer A、High pressure、Sonication or sample buffer lysis. Sera were obtained from patients with Lyme disease（n=21）,EM（n＝24）, healthy control subjects who had never Lyme disease（n＝76）, RA（n＝91）, AS（n＝48）, SLE（n＝47）, Söjogren’s
syndrome（n＝45）, and syphilis（n＝7）. Reactivity to B. burgdorferi strain B31 antigens were analyzed by Western blotting.
RESULT：The preparation method of Sonication is better than others. As in our study, Western blotting showed the major protein bands at 93、84、74、66、58、48、45、41、39、34 and 31kDa. The most prominent IgM response in patients with Lyme disease was to 41kDa（57％）、66kDa（33％）、39kDa（29％） and 35kDa（23％）proteins. The most prominent IgG response in patients with Lyme disease was to41kDa（100％）、74kDa（71％）、93kDa（57％）、58kDa（52％） and 45kDa（52％）. Four of 47 patients with SLE showed IgM positive immunoblot in Lyme disease and other diseases showed cross-reactivity to multiple Borrelia antigens.
In CONCLUSION： The preparation method of Sonication is better than others. The cross-reactive of SLE sera can cause the false-diagnosis of Lyme disease from patients with SLE. The results demonstrate the differences of the antigenic heterogeneity exist among the different genospecies, which in agreement with previous observations.
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