| Summary: | 碩士 === 中原大學 === 醫學工程研究所 === 92 === Traditional Chinese medicine attaches importance to research in international development and in recent years concentrated dosage form have been widely adopted for clinical use, which have few side effects. Berberine is an alkaloid that is derived from Coptis chinensis. It has a lot of pharmaceutical efficacy such as antibacterical, antifungal, treated hypertension, hyperlipemia, arrhythmia, diabetes mellitus and antiplatelet effect, etc. Recently, many literatures reported to process anti-tumor promoting activities effects so berberine attaches importance application at the pharmacology.
In this study, the SnO2/ITO glass as a pH sensor is used to detect berberine (BE) for traditional Chinese medicine. It is a solid-state sensor, which has a lot of advantages, such as small size, low cost and easy manufacture. The research includes two methods. First, acetylcholinesterase (AchE) is immobilized on the substrate to fabricate an enzyme field effect transistor (EnFET). Secondly, the pH sensor, which is modified with silicotungstic acid (STA; H4O40SiW12) and polyvinylchloride (PVC) is presented the poly vinyl chloride membrane field effect transistor (PVC membrane FET). The PVC membrane FET have detection linear range 1×10-3~ 5×10-7mol/L； the detection linear range of EnFET is 1×10-3~ 1×10-6mol/L and detection limit about 1×10-7mol/L. Compare PVC membrane FET with EnFET, PVC membrane FET has lower cost, better sensitivity, shorter response time and easier manufacture. So PVC membrane FET is better choice for berberine sensor than EnFET.
|
|---|
