Studies on the Production and Purification of Intracellular and Extracellular Polysaccharides from Grifola frondosa by Submerged Fermentation

• Main Authors: Cing-Yuan You, 游清源
• Other Authors: Tal-Hao Hsu
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/10845876459682177090

碩士 === 大葉大學 === 生物產業科技學系碩士班 === 92 === Grifola frondosa have been reported in many research articles include cure of edema, beriberi, ascites, hypertension and adiposis. Grifola frondosa’s polysaccharide is a β-(1-3)-linked glucan with branches of β-(1-6)-D-linked glucan showing the main of antitumor activity. Submerged fermentation of great advantage to cycle brevity, productive pregnant. Because of medicine fungus breed is biodiversity, then have different optimize cultured control factor, need respective to proceed systematized experimental. This study on the mycelial biomass growth and extracellular Polysaccharides from Grifola frondosa by continuous stirred tank reactor and response surface methodology. Production pure polysaccharides by purification of extracellular polysaccharides. The study shows that, in different cultured control experimental, best cultured control is achieved under the condition of 1.5% fructose, medium pH 4, cluture temp for 25℃; in steepest ascent path experimental, the highest mycelial biomass growth is achieved under the condition of 1.65% fructose, medium pH 4.01, cluture temp for 25.3℃, diffluence oxygen 71.93%; in different fermentation culture experimental, the highest mycelial biomass growth by continuous stirred tank reactor in 5L fermenter; the highest extracellular polysaccharides by 5L batch fermenter. All wave crest to have β-D-glucan in extracellular polysaccharides purification by ion exchange chromatography, all wave crest and β-D-glucan concentration is directly_proportional; coarse extracellular polysaccharides’s β-D-glucan concentration for 31.68 %.