Analysis of glycine and oligoglycines with low-energy He-Ne laser-induced fluorescence detector for capillary electrophoresis

• Main Authors: Hsieh Hsin Cheng, 謝欣成
• Other Authors: Chen Show Chuen
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/57837707464518185012

碩士 === 輔仁大學 === 化學系 === 92 === CE-LIF, capillary electrophoresis coupled with laser-induced fluorescence detection, is extremely sensitive. For glycine and its peptides after derivatized with 5-TRITC, Tetramethylrhodamine-5-isothiocyanate, it could sense a peptide at a low 9.3 nM. But the molecular size of derivatizing reagent overshadows its resolution power to result in unresolved peaks between tetraglycine and hexaglycine. Further development with modification of separation medium using acetonitrile or dimethyl sulfoxide has resolved all six peaks. Real sample of artificial sweetner was analyzed to demonstrate its feasibility.