The Effect of Bupleurum kaoi AgainstDimethylnitrosamine-Induced Hepatic Fibrosis in Rat

• Main Authors: Ting-Chun Weng, 翁鼎鈞
• Other Authors: Chun-Ching Lin
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/84205259961996976370

碩士 === 高雄醫學大學 === 天然藥物研究所碩士班 === 92 === Bupleurum kaoi Liu, Chau et Chuang, an endemic plant in Taiwan and one specie of Bupleuri Radix, is one of the most important traditional Chinese crude drugs for treating hepatitis and hepatoprotection. The three bioactive fractions-water extract (BKW), fraction of neutral polysaccharide (BKP) and saponin-enriched fraction (BKS) separated from B. kaoi, and the water extract (BCW), fraction of neutral polysaccharide (BCP) and saponin-enriched fraction (BCS) separated from B. chinense D.C. (as the reference plant) were examined effects against dimethylnitrosamine (DMN)-induced hepatic fibrosis in rats for 4 weeks. During last two weeks, rats in the liver injury model were daily administrated p.o. by the various doses of six fractions and silymarin as a reference compound. The liver anti-inflammation and fibrosis-improving effects of treatments were evaluated according to albumin, total protein and collagen in liver; L-asparate aminotransferase, triglyceride, albumin, total protein, interferon-γ (IFN-γ) and interleukin-10 (IL-10) in serum. Taken together, B. kaoi certainly exhibited potency of hepatoprotection and anti-inflammation, and the three fractions of B. kaoi further exhibited anti-hepatic fibrosis activities concerned with regulations of IFN-γ and IL-10.