| Summary: | 碩士 === 高雄醫學大學 === 天然藥物研究所碩士班 === 92 === The MeOH extract of leaves of Neolitsea buisanensis Yamamoto & Kamikoti f. buisanensis (Lauraceae) showed cytotoxicities against A549 (ED50 = 12.2 mg/ml), P-388 (ED50 = 2.0 mg/ml) cancer cell lines in vitro.
The investigation of chemical constituents in the 90% MeOH-soluble fraction from CHCl3-soluble fraction led to the isolation of 14 compounds, including two eudesmane-type sesquiterpenes: neobuisanolide A (2), neolitacumone B (12), one guaiane-type sesquiterpene: neobuisanolide B (4), four heliangolid-type sesquiterpenes: glechomanolid (3), neobuisanolide C (5), litseacassifolide (7), neobuisanolide D (8), five aromadendrane-type sesquiterpenes: spathulenol (1), (-)-ent-4b-hydroxy-10b-methoxyaromadendrane (9), (6b,7b)-4b-hydroxy-10a-methoxyaromadendrane (10), (6a,7a)-4b- hydroxy-10a-methoxyaromadendrane (11), neobuisanolide E (13), one aporphine-type alkaloid：N-formylanonaine (6), together with one ionone-type: 1-(3-hydroxy-but-1-enyl)-2,2,6-trimethyl-cyclohexane- 1,4-diol (14). Among these isolates, neobuisanolide A (2), B (4), C (5), D (8) and E (13) are new compounds from nature. (-)-ent-4b-Hydroxy-10b-methoxyaromadendrane (9) is first obtained in (-)-form, though the (+)-form has been previously reported. From n-BuOH-soluble fraction obtained five compounds, including neolitcarboline A (15), kaempferol-3-O-rhamonside (16), engelitin (17), taxifolin-3-O-rhamnoside (18) and isoastilbin (19). Among them, neolitcarboline A (15) is a new compound from nature. The structures of these isolates were determined by spectroscopic data.
Compounds 3, 12, 15 exhibited significant cytotoxicity, and 2, 6, 8 had marginal cytotoxicity against P-388 cancer cell line in vitro. Compounds 3, 6, 12, 15 showed marginal cytotoxicity against HT-29 cancer cell lines in vitro.
|
|---|
