| Summary: | 碩士 === 高雄醫學大學 === 生物化學研究所碩士班 === 92 === To explore more hNinein interacting proteins, the yeast
two-hybrid screening using ninein C-terminal domain as bait protein
was performed. One novel gene, CGI-99, was demonstrated to
associate with hNinein in the yeast two-hybrid method and in vitro
GST pull-down assay. Molecular characterization also showed that
CGI-99 possessed a transcriptional activity at the N-terminal. In
addition, CGI-99 formed a dimer with the C-terminal, which
overlapped with hNinein binding site. In kinase assay, CGI-99 binds
to hNinein completely blocks the phosphorylation of hNinein by
GSK3β. Moreover, CGI-99 was highly expressed in all brain tumors
which is in agreement with the northern blot analysis. Taken together,
we have isolated a novel protein CGI-99, which may be involved in
the function regulation of human ninein in the centrosome structure
and may also be important in brain development and tumorigenesis.
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