1. Quantitative Determination of Indomethacin in Human Plasma by Micellar Electrokinetic Chromatography2. Simultaneous Determination of Cefepime and l-Arginine by Micellar Electrokinetic Chromatography

• Main Authors: Yi-Ru Chen, 陳薏如
• Other Authors: Su-Hwei Chen
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/53586525060309400837

碩士 === 高雄醫學大學 === 藥學研究所碩士班 === 92 === The purpose of this work is directed to the development of accurate and sensitive methods for the different medicines by micellar electrokinetic chromatography. The main study included analysis of indomethacin in human plasma and simultaneous determination of cefepime and l-arginine by micellar electrokinetic chromatography. The results are summarized as follow： 1. Quantitative Determination of Indomethacin in Human Plasma by Micellar Electrokinetic Chromatography. A simple micellar electrokinetic chromatography is described for determination of indomethacin in human plasma. The analysis of indomethacin in human plasma was performed in Tris buffer (30 mM; pH 8) with 100 mM octane-1-sulfonic acid sodium salt as a background electrolyte. Several parameters affecting the separation of the drug were studied, including the pH and concentrations of the Tris buffer and octane-1-sulfonic acid sodium salt. Application of the method to the determination of indomethacin in human plasma proved to be feasible. 2. Simultaneous Determination of Cefepime and l-Arginine by Micellar Electrokinetic Chromatography A simple micellar electrokinetic chromatography is described for simultaneous determination of cefepime and l-arginine. The method was performed in Tris buffer (10 mM; pH 8.0) with 80 mM sodium dodecyl sulfate as a background electrolyte. Several parameters affecting the separation of the drugs were studied, including the pH and concentrations of the Tris buffer and sodium dodecyl sulfate. Application of the proposed method to the determination of cefepime and l-arginine in commercial injections proved to be feasible.