Anti-allergic effect of milk fermented with lactic acid bacteria

• Main Authors: Peng Hsuan Ti, 彭瑄第
• Other Authors: Meei-Yn Lin, Ph. D.
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/21497158728372527484

碩士 === 國立中興大學 === 食品科學系 === 92 === The purpose of this investigation was to assess the anti-allergic effect of fermented milk of Streptococcus thermophilus MC, Lactobacillus acidophilus B, Lactobacillus bulgaricus Lb, Lactobacillus bulgaricus 448, and Bifidobacterium longum B6. Female BALB/c mice were immunized intraperitoneally with ovalbumin/complete Freund’s adjuvant to evaluate the specific immune response of mice fed with fermented milk by judging from cytokine, antibody and nitric oxide analysis. Both cultures of spontaneous and OVA-stimulated splenocytes from mice fed with L. bulgaricus Lb of fermented milk expressed significant reduction of IL-4 and promotion of IFN-γ secretion as compared with milk-fed controls. The IL-4 of spontaneous secretion of splenocytes from mice fed with milk fermented with S. thermophilus MC or L. acidophilus B was significantly decreased. When mice fed milk fermented with L. acidophilus B, L. bulgaricusm 448 or B. longum B6, the secretion of IL-6 from cultures of spontaneous or LPS-stimulated peritoneal cells significantly decreased. All tested LAB decreased the secretion of TNF- of LPS-stimulated peritoneal cells from OVA/CFA-primed mice (P < 0.05) and demonstrated the effect of anti-inflammation. The milk fermented with S. thermophilus MC was able to significantly stimulate the secretion of NO from peritoneal cell of mice by activating macrophage. The ratio of IFN-γ to IL-4 of spontaneous secretion of splenocytes, milk fermented with L. bulgaricus Lb significantly increased. The result of anti-OVA-IgE antibody from serum of OVA/CFA-primed mice indicated that S. thermophilus MC is more anti-allergic than that of milk fermented with other tested strains.