Changes in the morphology and Components of Pig Follicles and Oocytes During Folliculogenesis

• Main Authors: Chii-Ming Chiou, 邱啟明
• Other Authors: Mu-Chiou Huang
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/46048128243024201337

博士 === 國立中興大學 === 畜產學系 === 92 === Changes in the morphology and Components of Pig Follicles and Oocytes During Folliculogenesis The purpose of this study was to investigate the variation of number of granulosa cells, and diameter of oocyte obtained from different development stage of follicles. The amounts of follicular fluid were also measured. The follicles were classified into four groups, according to their diameter, ＜3.0 (small, S), 3.1-5.0 (medium-1, MI), 5.1-7.0 (medium-2, MII) and ＞7.1 (large, L) mm. There were significant differences (p<0.05) in the number of follicle between follicle size and non-atretic and atretic follicles per ovary, respectively. The numbers of granulosa cells in non-atretic and atretic follicles were also showed in the same results. The diameter of preantral follicles was classified into three classes, 200＜300 (small preantral, SP), 301＜400 (medium preantral, MP), and 401＜500 (large preantral, LP)μm to investigate the correlation between diameter of preantral follicles and oocytes of porcine. The results showed that diameters of oocyte in SP, MP and LP follicles increased according to the follicle diameters. Investigation of the correlation between the diameter of follicles and oocytes was carried out. The diameters of the lacking or containing zona pellucida oocyte in non-atretic and atretic follicles, between follicle size has a significant difference (p＜0.05). However, average diameter of containing cumulus oophorus oocyte (COCs) in non-atretic and atretic follicles, between follicle size and follicle class has a significant difference (p＜0.05). The results suggested that mean of follicular fluid content and number of granulosa cells in both non-atretic and atretic follicles increased when the diameter of follicles increased. Diameter of oocyte increased significantly at preantral stage when antral formed. Once diameter of follicle reached 5 mm, thereafter diameter of oocyte increased slowly. Number of granulosa cells obtained from atretic follicles was lower than that of non-atretic, but follicular fluid content and diameter of COCs was inversed. We hope these results might provide valuable information in selecting better follicles or oocytes for culture in vitro. The results showed that the average levels of estradiol-17β(E2) were 6.4 ±0.63 vs. 3.2 ±0.27,10.6 ±0.32 vs. 3.2 ±0.47,47.6 ±1.10 vs. 47.4 ±1.25 and 70.0 ±3.32 vs. 30.1 ±1.98 ng/ml (p＜0.05) in the S, MI, MII and L groups, respectively. In the MII follicles, the level of E2 in both the non-atretic and atretic groups was similar. A very low concentration of E2 was observed in the cyst follicles. The P4 levels in the four groups of the non-atretic and atretic follicles were 56.6 ±4.7 vs. 46.3 ±3.9﹑82.1 ±7.0 vs. 59.1 ±4.5﹑203.3 ±34.5 vs. 51.2 ±3.8 and 326.1 ±56.3 vs. 668.1 ±85.7 ng/ml, respectively. Very high concentrations of P4 were observed in the cyst follicles. The concentrations of testosterone (T) were 9.2 ±5.3 vs. 13.9 ±5.9 , 24.2 ±1.8 vs. 38.3 ±2.3 , 177.6 ±3.8 vs. 74.1 ±2.1 and 120.5 ±14.0 vs. 204.3 ±18.2 ng/ml, respectively. Low concentrations of T were found in the cyst follicles. The levels of FSH and LH between non-atretic and atretic follicles were similar, but the accompanying increase in follicle size tended to be smaller. Follicular fluid and oocyte and granulosa cells were used by 2-D SDS electrophoresis gel assay. The band density of protein content in follicular fluid obtained from non- atretic follicles were less then atretic follicles in S and MI group. However, the band density of protein content in follicular fluid obtained from non- atretic follicle was higher then atretic follicle in MII and S group. The band density of protein content of oocyte, that obtained from non-atretic follicles were higher then obtained from atretic follicles in S group. However, obtained from non-atretic follicles were less then obtained from atretic follicles in L group. Band of protein content of electrophoresis graph in granulosa cells, that obtained from atretic follicles were more a band then obtained from non-atretic follicles in S group. However, the band density of protein content of electrophoresis graph in granulosa cells, obtained from atretic follicles were higher then obtained from non-atretic follicles in L group.