| Summary: | 碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 92 === Abstract
The object of this research is titanium, a metal material of synthetic implant, and this research evaluates respectively the three categories of (1) the nature of the material, (2) the material and cell culture, and (3) the material and organ culture. Having been etched, the surface layer of titanium has mm-sized beehive-like coarse holes, the roughness of which can be as big as 2.28μm, which is bigger than the holes commonly seen on the etching surface. We can find in the analysis on the component parts of the surface layer that the layer is more oxidized in TA than in TM. In cell adhesion and growth, we find that it is non-poisonous and bio-compatible. Through the SEM observation we find that the cell morphology is smoother in TA than in TM and there are more filopodia attached; therefore, the mechanical interlocking between the implant surface and osseous tissues can be expected to be enhanced. In organ culture, the frontal head bone of the newborn mouse is cultured with TM and TA, and we observed the growth and calcification of the defected bone tissue on the rims of the holes. Through the contra-photomicroscope, we observed that TA and TM are osteoconductive and that the bone cells attached themselves to the rim of the material from the rims of the holes. Furthermore, through the SEM observation and quantitative analysis, we found that, the organ having been cultured for five weeks, there were more and thicker cells on the bottom, side and surface of the TA than on those of TM, and the calcification of TA is more obvious than that of TM. To sum up, TA is an ideal implant material.
Key words：titanium, dental implant, osteoprogenitor cells, osseointegration, biocompatibility
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