To Design the Creatinine Imprinted Polymers Based on the Information from Microcalorimeter

• Main Authors: Wei-Chih Chen, 陳威志
• Other Authors: Tse-Chuan Chou
• Format: Others
• Language: zh-TW
• Published: 2004
• Online Access: http://ndltd.ncl.edu.tw/handle/yeu6ya

碩士 === 國立成功大學 === 化學工程學系碩博士班 === 92 === 　　The creatinine concentrations in serum or urine are very important determinants to help doctors to diagnose whether a patient’s kidney function is satisfactory. Consequently, there are many researchers trying to develop improving methods to quantify the exact concentration of creatinine in serum and urine due to the non-specific of traditional method to measure the conc. of creatinine. In this work, three different experiments were performed, and we tried to explain the binding mechanism between creatinine and functional monomer and how to select a monomer to prepare a creatinine MIP with high selectivity. First, the pH of several functional monomers, creatine, and creatinine aqueous solution were measured. The result shows MAA is more acidic than HEMA or NVP, and creatinine is a basic compound. So we can predict an MAA based MIP will interact with creatinine more strongly than HEMA and NVP based MIPs. Functional monomers were titrated to creatinine particles and creatinine solutions in order to understand the interaction between functional monomers and creatinine. The isothermal titration confirmed the interaction between MAA and creatinine was stronger than HEMA and NVP. We also found how many functional monomer molecules will interact with one creatinine at 25℃ by accumulating the heat response from the titrating process of each functional monomer and the critical point of the titration process. There were 2.14 MAA monomer molecules interacting with one creatinine. For NVP and HEMA the binding ratios were 2.8 and 3.38, respectively. So if the HEMA in the complex of HEMA and creatinine was fixed to form a MIP at 25℃, the selectivity of this MIP would be better than another functional monomer based MIP due to the larger ratio of HEMA/Crn at the critical point. Finally, MIP particles and micro-contact MIP films were prepared to examine the real binding mechanism. Results showed the amount of creatinine adsorbed by the MAA based MIP was higher than that of the other functional monomer based MIPs. The non-imprinted polymer particles still adsorbed almost same amount of creatinine with respect to the imprinted polymer particles. The selectivity of the MAA based MIP was better than that of the HEMA based MIP. This is because the complex formed by MAA and creatinine may not have been destroyed at the high polymerizing temperature and MAA can recognize basic materials due to the nature of acid-base complement. We also prepared a micro-contact MIP film on two glass surfaces and managed to reduce the amount of adsorption by the non-imprinted polymer films.