The Reactions of 3-(N-Methylamino)methyl Catechol and 3-Aminomethyl Catechol with Metapyrocatechase
碩士 === 國立成功大學 === 化學系碩博士班 === 92 === The results of cell culture experiments using semi-continuous fermentation system indicated that the benzoate is the limited species of the growth media. Another limited of growth factor is the oxygen supply. When the benzoate concentration was 9 g/L and the...
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ndltd-TW-092NCKU50650382019-05-15T20:21:37Z http://ndltd.ncl.edu.tw/handle/fu7q22 The Reactions of 3-(N-Methylamino)methyl Catechol and 3-Aminomethyl Catechol with Metapyrocatechase 3-(N-甲基胺基)甲基兒茶酚及3-胺基甲基兒茶酚與偏鄰苯二酚雙加氧酶的反應 Wei-Hsu Lai 賴韋旭 碩士 國立成功大學 化學系碩博士班 92 The results of cell culture experiments using semi-continuous fermentation system indicated that the benzoate is the limited species of the growth media. Another limited of growth factor is the oxygen supply. When the benzoate concentration was 9 g/L and the air supply was 5 L/min with the agitation speed of 600 rpm, a high yield (15g/L) of Pseudomonas cells can be obtained. The Ki’s of 3-chlorocatechol, 3-aminomethyl catechol and 3-(N-methylamino)methyl catechol against metapyrocatechase were 1.86μM, 65.98μM and 85.48μM, respectively. The Km’s of 3-aminomethyl catechol and 3-(N-methylamino)methyl catechol are 53.94μM and 70.93μM, respectively. These results indicated that the inhibition ability of the substituted catechol is government by the size of substituent in the 3-position of catechol. The inactivation constant of 3-aminomethyl catechol and 3-(N-methylamino)methyl catechol was also examined. The Kinact of these two inactivators are 0.24 min-1 and 0.20 min-1, respectively. Ded-Shih Huang 黃得時 2004 學位論文 ; thesis 86 zh-TW |
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碩士 === 國立成功大學 === 化學系碩博士班 === 92 === The results of cell culture experiments using semi-continuous fermentation system indicated that the benzoate is the limited species of the growth media. Another limited of growth factor is the oxygen supply. When the benzoate concentration was 9 g/L and the air supply was 5 L/min with the agitation speed of 600 rpm, a high yield (15g/L) of Pseudomonas cells can be obtained.
The Ki’s of 3-chlorocatechol, 3-aminomethyl catechol and 3-(N-methylamino)methyl catechol against metapyrocatechase were 1.86μM, 65.98μM and 85.48μM, respectively. The Km’s of 3-aminomethyl catechol and 3-(N-methylamino)methyl catechol are 53.94μM and 70.93μM, respectively. These results indicated that the inhibition ability of the substituted catechol is government by the size of substituent in the 3-position of catechol.
The inactivation constant of 3-aminomethyl catechol and 3-(N-methylamino)methyl catechol was also examined. The Kinact of these two inactivators are 0.24 min-1 and 0.20 min-1, respectively.
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author2 |
Ded-Shih Huang |
author_facet |
Ded-Shih Huang Wei-Hsu Lai 賴韋旭 |
author |
Wei-Hsu Lai 賴韋旭 |
spellingShingle |
Wei-Hsu Lai 賴韋旭 The Reactions of 3-(N-Methylamino)methyl Catechol and 3-Aminomethyl Catechol with Metapyrocatechase |
author_sort |
Wei-Hsu Lai |
title |
The Reactions of 3-(N-Methylamino)methyl Catechol and 3-Aminomethyl Catechol with Metapyrocatechase |
title_short |
The Reactions of 3-(N-Methylamino)methyl Catechol and 3-Aminomethyl Catechol with Metapyrocatechase |
title_full |
The Reactions of 3-(N-Methylamino)methyl Catechol and 3-Aminomethyl Catechol with Metapyrocatechase |
title_fullStr |
The Reactions of 3-(N-Methylamino)methyl Catechol and 3-Aminomethyl Catechol with Metapyrocatechase |
title_full_unstemmed |
The Reactions of 3-(N-Methylamino)methyl Catechol and 3-Aminomethyl Catechol with Metapyrocatechase |
title_sort |
reactions of 3-(n-methylamino)methyl catechol and 3-aminomethyl catechol with metapyrocatechase |
publishDate |
2004 |
url |
http://ndltd.ncl.edu.tw/handle/fu7q22 |
work_keys_str_mv |
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