| Summary: | 碩士 === 國立成功大學 === 生物科技研究所碩博士班 === 92 === Many viruses can infect their hosts as both productive and persistent infections. Persistently infected viruses can be reactivated when the host surveillance is decreased. It is an important issue to study the mechanism of the viral persistence. Hz-1 virus is a good model virus for that it is an insect virus and cannot infect human being. In addition, Hz-1 virus can establish persistently infected cell lines. Thus, Hz-1 virus is a very useful model virus for the study of persistent infection.
This proposal was to study the kinetics of gene expressions of Hz-1 virus during productive and persistent infections and to study the possible functions of these genes. Therefore, biochip technology was applied to establish Hz-1 viral gene expression profile during productive and persistent infections. Genes with similar functions have the same expression patterns. Using cluster analysis, all productively expressed genes were classified into six groups according to their similar expression patterns. Comparing genes with known function to those with unknown functions, the possible functions of these unknown genes were assigned. In addition, all Hz-1 viral ORFs that expressed during the persistent infection could be divided into six groups. The expression profiles of the Hz-1 virus during its productive and persistent infections were confirmed with RT-PCR and northern blot analyses.
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